从余甘子中克隆诱导细胞凋亡的 novel lectin cDNA。
cDNA cloning of a novel lectin that induce cell apoptosis from Artocarpus hypargyreus.
机构信息
Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning 530021, China.
Department of Orthopaedics, Chinese and Western Orthopaedics Hospital of Guigang, Guigang 537100, China.
出版信息
Chin J Nat Med. 2021 Feb;19(2):81-89. doi: 10.1016/S1875-5364(21)60009-2.
We isolated a novel lectin (AHL) from Artocarpus hypargyreusHance and showed its immunomodulatory activities. In this study, the amino acid sequence of AHL was determined by cDNA sequencing. AHL cDNA (875bp) contains a 456-bp open reading frame (ORF), which encodes a protein with 151 amino acids. AHL is a new member of jacalin-related lectin family (JRLs), which share high sequence similarities to KM+ and Morniga M, and contain the conserved carbohydrate binding domains. The antitumor activity of AHL was also explored using Jurkat T cell lines. AHL exhibits a strong binding affinity to cell membrane, which can be effectively inhibited by methyl-α-D-galactose. AHL inhibits cell proliferation in a time- and dose-dependent manner through apoptosis, evidenced by morphological changes, phosphatidylserine externalization, poly ADP-ribose polymerase (PARP) cleavage, Bad and Bax up-regulation, and caspase-3 activation. We further showed that the activation of ERK and p38 signaling pathways is involved for the pro-apoptotic effect of AHL.
我们从 Artocarpus hypargyreus Hance 中分离出一种新型凝集素（AHL），并显示其免疫调节活性。在这项研究中，通过 cDNA 测序确定了 AHL 的氨基酸序列。AHL cDNA（875bp）包含一个 456bp 的开放阅读框（ORF），编码一个含有 151 个氨基酸的蛋白质。AHL 是 jacalin 相关凝集素家族（JRLs）的新成员，与 KM+和 Morniga M 具有高度的序列相似性，并包含保守的碳水化合物结合结构域。还使用 Jurkat T 细胞系探索了 AHL 的抗肿瘤活性。AHL 对细胞膜具有很强的结合亲和力，可被甲基-α-D-半乳糖有效抑制。AHL 通过凋亡以时间和剂量依赖的方式抑制细胞增殖，这可以通过形态变化、磷脂酰丝氨酸外翻、多聚 ADP-核糖聚合酶（PARP）切割、Bad 和 Bax 上调以及 caspase-3 激活来证明。我们进一步表明，ERK 和 p38 信号通路的激活参与了 AHL 的促凋亡作用。
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