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通过抗氧化机制推断简便合成的蓝藻银纳米颗粒的抗菌功效

Antibacterial efficacy of facile cyanobacterial silver nanoparticles inferred by antioxidant mechanism.

作者信息

Husain Shaheen, Verma Suresh K, Azam Mudsser, Sardar Meryam, Haq Q M R, Fatma Tasneem

机构信息

Cyanobacterial Biotechnology Lab, Department of Biosciences, Jamia Millia Islamia, New Delhi, India.

School of Biotechnology, KIIT University, Bhubaneswar, India.

出版信息

Mater Sci Eng C Mater Biol Appl. 2021 Mar;122:111888. doi: 10.1016/j.msec.2021.111888. Epub 2021 Jan 15.

Abstract

Nanoparticles (NPs) have gained importance in technological advances owing to their user friendly enhanced and efficient physical, chemical, and biological characteristics compared to their bulk counterparts. Biological synthesis of NPs by using a microorganism, enzymes, or plant extracts offers a greener and eco-friendly approach besides many advantages over physical or chemical approaches. This study reports the biosynthesis of silver nanoparticles (AgNPs) using Nostoc muscorum NCCU 442 aqueous extract as the reducing and capping agent for AgNPs synthesis. The synthesized nanoparticles were characterized by UV-VIS spectrum, SEM, EDS, TEM, AFM, DLS and XRD. Results showed distinguishing polycrystalline nature of synthesized AgNPs with surface plasmon significant band in the size range of 6-45nm with average 30 size nm. FT-IR study revealed the role of secondary metabolites present in aqueous extract for the synthesis of AgNPs. Biological activities of purified AgNPs as antioxidant and antibacterial potential showed the highest antibacterial activity against Staphylococcus aureus MTCC 902.

摘要

由于纳米颗粒(NPs)相较于其块状对应物具有用户友好的增强且高效的物理、化学和生物学特性,因此在技术进步中变得愈发重要。利用微生物、酶或植物提取物进行NPs的生物合成,除了比物理或化学方法具有许多优势外,还提供了一种更绿色环保的方法。本研究报道了使用地木耳NCCU 442水提取物作为AgNPs合成的还原剂和封端剂来生物合成银纳米颗粒(AgNPs)。通过紫外可见光谱、扫描电子显微镜、能谱分析、透射电子显微镜、原子力显微镜、动态光散射和X射线衍射对合成的纳米颗粒进行了表征。结果表明,合成的AgNPs具有独特的多晶性质,表面等离子体显著带在6 - 45nm尺寸范围内,平均尺寸为30nm。傅里叶变换红外光谱研究揭示了水提取物中存在的次生代谢产物在AgNPs合成中的作用。纯化的AgNPs的生物活性作为抗氧化剂和抗菌潜力显示出对金黄色葡萄球菌MTCC 902具有最高的抗菌活性。

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