从莫斯科地区患乳腺炎奶牛中分离出的微生物在生物膜形成中的作用。
Role of microorganisms isolated from cows with mastitis in Moscow region in biofilm formation.
作者信息
Rudenko Pavel, Sachivkina Nadezhda, Vatnikov Yury, Shabunin Sergey, Engashev Sergey, Kontsevaya Svetlana, Karamyan Arfenia, Bokov Dmitry, Kuznetsova Olga, Vasilieva Elena
机构信息
Biological Testing Laboratory, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow Region, Russia.
Department of Veterinary Medicine, Peoples' Friendship University of Russia, Moscow, Russia.
出版信息
Vet World. 2021 Jan;14(1):40-48. doi: 10.14202/vetworld.2021.40-48. Epub 2021 Jan 7.
BACKGROUND AND AIM
Mastitis is one of the most important diseases of cows and the most expensive pathology for the dairy industry. Therefore, this study was conducted to explore the role of microorganisms isolated from cows with mastitis in the formation of biofilms under the conditions of farm biogeocenosis in the Moscow region.
MATERIALS AND METHODS
Periodic visits to 12 farms in the Moscow region were conducted to explore the microbial profile of the udder of cows with mastitis. During the visits, 103 milk samples from sick animals were collected and examined. Through microbiological analyses, 486 cultures of microorganisms were identified, which are assigned to 11 genera. Mastitis in cows is caused not only by a single pathogen but also by microbial associations, which included two to seven microbial isolates.
RESULTS
It was observed that 309 isolates (63.6%) from the total number of isolated microorganisms could form a biofilm. The ability to form biofilms was most frequently observed in (18.8%), (11.9%), and (11.7%) cultures from the total number of biofilm-forming microbial cultures. Low biofilm-forming ability among the isolated microorganisms was found in lactobacilli, wherein only 20 (22.5%) strains had the ability to form biofilms. The isolated microorganisms exhibited different sensitivities to antimicrobial agents, which cause difficulty in selecting an antimicrobial agent that would act on all aspects of the parasitocenosis.
CONCLUSION
A high proportion of microorganisms isolated from cows with mastitis have the ability to form biofilms. The isolated microorganisms exhibited different and highly heterogeneous sensitivity to the action of antimicrobial drugs. This causes difficulty in using these tools for the effective control of mastitis in cows, which is frequently caused by pathogenic associations of microbial biofilms. Therefore, it is important to explore novel and more effective methods to combat this disease.
背景与目的
乳腺炎是奶牛最重要的疾病之一，也是乳制品行业最昂贵的病理问题。因此，本研究旨在探讨从患有乳腺炎的奶牛中分离出的微生物在莫斯科地区农场生物地球群落条件下生物膜形成中的作用。
材料与方法
定期走访莫斯科地区的12个农场，以探究患有乳腺炎奶牛乳房的微生物概况。走访期间，收集并检查了103份患病动物的牛奶样本。通过微生物学分析，鉴定出486种微生物培养物，它们分属于11个属。奶牛乳腺炎不仅由单一病原体引起，还由微生物联合体引起，其中包括两到七种微生物分离株。
结果
观察到，在分离出的微生物总数中，有309株（63.6%）能够形成生物膜。在形成生物膜的微生物培养物总数中， （18.8%）、 （11.9%）和 （11.7%）的培养物最常观察到形成生物膜的能力。在分离出的微生物中，乳酸菌形成生物膜的能力较低，其中只有20株（22.5%）菌株具有形成生物膜的能力。分离出的微生物对抗菌剂表现出不同的敏感性，这使得难以选择一种能作用于寄生群落各个方面的抗菌剂。
结论
从患有乳腺炎的奶牛中分离出的微生物中有很大比例具有形成生物膜的能力。分离出的微生物对抗菌药物的作用表现出不同且高度异质的敏感性。这使得使用这些工具有效控制奶牛乳腺炎变得困难，而奶牛乳腺炎通常是由微生物生物膜的致病联合体引起的。因此，探索对抗这种疾病的新颖且更有效的方法很重要。
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