鸡生长激素受体基因环状RNA对细胞增殖的影响
Effects of Circular RNA of Chicken Growth Hormone Receptor Gene on Cell Proliferation.
作者信息
Xu Haidong, Leng Qiying, Zheng Jiahui, Adu-Asiamah Patricia, Lin Shudai, Li Ting, Wang Zhang, An Lilong, Zhao Zhuihui, Zhang Li
机构信息
College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, China.
Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University, Guangzhou, China.
出版信息
Front Genet. 2021 Feb 11;12:598575. doi: 10.3389/fgene.2021.598575. eCollection 2021.
Animal growth and development are regulated by neural and endocrine growth axes, in which cell proliferation plays key roles. Recently, many research showed that circular RNAs were involved in hepatocyte and myoblast proliferation. Previously, we identified a circular RNA derived from the chicken gene, named circGHR. However, the function of circGHR is unclear. The objective of this study was to investigate circGHR expression pattern and its roles in cell proliferation. Results indicated that circGHR was a closed-loop structure molecule, and it was richer in the nucleus of hepatocytes and myoblast. Real-time PCR showed that circGHR was increased from E13 to the 7th week in the liver but decreased in the thigh and breast muscle. The CCK-8 assay displayed that circGHR promoted cell proliferation. Simultaneously, the biomarker genes , , and and the linear transcripts and were upregulated when circGHR was overexpressed. Altogether, these data exhibited that circGHR could promote cell proliferation possibly by regulating mRNA and expression.
动物的生长和发育受神经和内分泌生长轴调控，其中细胞增殖起着关键作用。最近，许多研究表明环状RNA参与肝细胞和成肌细胞的增殖。此前，我们鉴定出一种源自鸡基因的环状RNA，命名为circGHR。然而，circGHR的功能尚不清楚。本研究的目的是探究circGHR的表达模式及其在细胞增殖中的作用。结果表明，circGHR是一种闭环结构分子，在肝细胞和成肌细胞核中含量更丰富。实时PCR显示，circGHR在肝脏中从胚胎第13天到第7周表达增加，但在大腿和胸肌中表达降低。CCK-8检测显示circGHR促进细胞增殖。同时，当circGHR过表达时，生物标志物基因、和以及线性转录本和上调。总之，这些数据表明circGHR可能通过调节mRNA和表达来促进细胞增殖。
Zotero 插件