Papadopoulos Angelos, Chalmantzi Varvara, Hyvönen Marko, Stellas Dimitris, Syrrou Marika, Fotsis Theodore, Murphy Carol
School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom.
Cardiovascular Division, King's College London British Heart Foundation Centre of Excellence, London, SE5 9NU, United Kingdom.
Data Brief. 2021 Feb 6;35:106844. doi: 10.1016/j.dib.2021.106844. eCollection 2021 Apr.
Human embryonic stem cells exhibit great potential as a therapeutic tool in regenerative medicine due to their self-renewal and trilineage differentiation capacity. Maintaining this unique cellular state has been shown to rely primarily on the Activin A / TGFβ signaling pathway. While most conventional culture media are supplemented with TGFβ, in the current study we utilize a modified version of the commercially available mTeSR1, substituting TGFβ for Activin A in order to preserve pluripotency. (1) Cells cultured in ActA-mTesR express pluripotency factors NANOG, OCT4 and SOX2 at comparable levels with cells cultured in TGFβ-mTeSR. (2) ActA-mTeSR cultured cells retain a physiological karyotype. (3) Cells in ActA-mTeSR maintain their trilineage differentiation capacity as shown in the teratoma formation assay. This system can be used to dissect the role of Activin A, downstream effectors and signaling cascades in human embryonic stem cell responses.
人类胚胎干细胞因其自我更新和三系分化能力，在再生医学中作为一种治疗工具展现出巨大潜力。维持这种独特的细胞状态主要依赖于激活素A/TGFβ信号通路。虽然大多数传统培养基都添加了TGFβ，但在本研究中，我们使用了市售mTeSR1的改良版本，用激活素A替代TGFβ以维持多能性。（1）在ActA-mTeSR中培养的细胞表达多能性因子NANOG、OCT4和SOX2的水平与在TGFβ-mTeSR中培养的细胞相当。（2）ActA-mTeSR培养的细胞保持生理核型。（3）ActA-mTeSR中的细胞如在畸胎瘤形成试验中所示，维持其三系分化能力。该系统可用于剖析激活素A、下游效应器和信号级联在人类胚胎干细胞反应中的作用。
