Guerrero Felix D, Ghaffari Noushin, Bendele Kylie G, Metz Richard P, Dickens C Michael, Blood Philip D, Tidwell Jason, Miller Robert J, de León Adalberto A Pérez, Teel Pete D, Johnson Charles D
USDA-ARS Knipling-Bushland US Livestock Insect Research Laboratory and Veterinary Pest Genomics Center, 2700 Fredericksburg Road, Kerrville, TX 78028, USA.
Department of Computer Science, Roy G. Perry College of Engineering, Prairie View A&M University, Prairie View, TX 77446, USA.
Data Brief. 2021 Feb 6;35:106852. doi: 10.1016/j.dib.2021.106852. eCollection 2021 Apr.
Ticks from the genus have enormous global economic impact as ectoparasites of cattle. and are known to harbor infectious pathogens such as , and . Having reference quality genomes of these ticks would advance research to identify druggable targets for chemical entities with acaricidal activity and refine anti-tick vaccine approaches. We sequenced and assembled the genomes of and , using Pacific Biosciences and HiSeq 4000 technologies on very high molecular weight genomic DNA. We used 22 and 29 SMRT cells on the Pacific Biosciences Sequel for and , respectively, and 3 lanes of the Illumina HiSeq 4000 platform for each tick. The PacBio sequence yields for and were 21.0 and 27.9 million subreads, respectively, which were assembled with Canu v. 1.7. The final Canu assemblies consisted of 92,167 and 57,796 contigs with an average contig length of 39,249 and 69,055 bp for and , respectively. Annotated genome quality was assessed by BUSCO analysis to provide quantitative measures for each assembled genome. Over 82% and 92% of the 1066 member BUSCO gene set was found in the assembled genomes of and , respectively. For , only 189 of the 1066 BUSCO genes were missing and only 140 were present in a fragmented condition. For , only 75 of the BUSCO genes were missing and only 109 were present in a fragmented condition. The raw sequencing reads and the assembled contigs/scaffolds are archived at the National Center for Biotechnology Information.
该属蜱作为牛的体外寄生虫,对全球经济有着巨大影响。已知 和 携带诸如 、 和 等传染性病原体。拥有这些蜱的参考质量基因组将推动研究,以确定具有杀螨活性的化学实体的可成药靶点,并完善抗蜱疫苗方法。我们使用太平洋生物科学公司(Pacific Biosciences)和HiSeq 4000技术,对非常高分子量的基因组DNA进行测序和组装,获得了 和 的基因组。我们分别在太平洋生物科学公司的Sequel上使用22个和29个SMRT细胞对 和 进行测序,每个蜱在Illumina HiSeq 4000平台上使用3条泳道。 和 的PacBio序列产量分别为2100万和2790万个子读数,使用Canu v. 1.7进行组装。最终的Canu组装分别由92167个和57796个重叠群组成, 和 的重叠群平均长度分别为39249 bp和69055 bp。通过BUSCO分析评估注释基因组质量,为每个组装基因组提供定量指标。在 和 的组装基因组中,分别发现了1066个成员BUSCO基因集中超过82%和92%的基因。对于 ,1066个BUSCO基因中只有189个缺失,只有140个处于片段化状态。对于 ,只有75个BUSCO基因缺失,只有109个处于片段化状态。原始测序读数和组装的重叠群/支架存档于美国国立生物技术信息中心。