Wang Xin-Cun, Zeng Zhao-Qing, Zhuang Wen-Ying
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, PR China.
Mitochondrial DNA B Resour. 2018 Oct 27;3(2):1147-1148. doi: 10.1080/23802359.2018.1522979.
The complete nucleotide sequence of the mitochondrial genome of the bambusicolous fungus was determined using the next-generation sequencing technology. The circular molecule is 63,593 bp long with a GC content of 31.92%. Gene prediction revealed 44 genes encoding 15 conserved proteins, 27 tRNAs, and the large and small ribosomal RNAs. All genes are located on the same strand. The tRNA genes contain codons for all 20 standard amino acids. It turns out to be similar to the previously sequenced mitochondrial genomes of and . The differences lie in the number of introns embodied in protein-coding genes. Four introns exist in the mitochondrial genome of , 10 in , and 14 in . The phylogenetic analysis confirmed as a member of (Nectriaceae). The mitochondrial genome of will contribute to the understanding of phylogeny and evolution of the genus and family.
利用下一代测序技术测定了竹生真菌线粒体基因组的完整核苷酸序列。该环状分子长63,593 bp，GC含量为31.92%。基因预测显示有44个基因，编码15种保守蛋白、27个tRNA以及大、小核糖体RNA。所有基因都位于同一条链上。tRNA基因包含所有20种标准氨基酸的密码子。结果表明它与之前测序的[具体物种1]和[具体物种2]的线粒体基因组相似。差异在于蛋白质编码基因中内含子的数量。[具体物种1]的线粒体基因组中有4个内含子，[具体物种2]中有10个，[目标物种]中有14个。系统发育分析证实[目标物种]是[科名]（丛赤壳科）的一个成员。[目标物种]的线粒体基因组将有助于理解该属和该科的系统发育和进化。
