Wang Xin-Cun, Wu Kai, Chen Haimei, Shao Junjie, Zhang Nana, Chen Xiangdong, Lan Jin, Liu Chang
a Institute of Medicinal Plant Development, Chinese Academy of Medical Science , Beijing , PR China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4197-4198. doi: 10.3109/19401736.2015.1022737. Epub 2015 Mar 27.
Complete nucleotide sequence of the 78,447 bp mitochondrial genome of the white-rotting fungus Ganoderma meredithiae Adask. & Gilb. has been determined by next-generation sequencing technology. The circular molecule encodes a set of mitochondrial protein and RNA genes, including 15 conserved proteins, 29 tRNAs, large and small ribosomal RNAs, and 18 homing endonucleases, with a GC content of 26.14%. All structural genes are located on the same strand except trnW-CCA. Compared with previously sequenced mtDNAs of G. lucidum and G. sinense, the gene order of protein and rRNA genes among the three mitogenomes is highly conserved; however, the tRNA composition is slightly different. The mitochondrial genome of G. meredithiae will contribute to understanding the phylogeny and evolution of Ganoderma and Ganodermataceae, the group containing many species with high medicinal values.
通过下一代测序技术已确定了白腐真菌梅雷迪灵芝(Ganoderma meredithiae Adask. & Gilb.)78,447 bp线粒体基因组的完整核苷酸序列。该环状分子编码一组线粒体蛋白质和RNA基因,包括15个保守蛋白质、29个tRNA、大核糖体RNA和小核糖体RNA以及18个归巢内切酶,GC含量为26.14%。除trnW-CCA外,所有结构基因都位于同一条链上。与先前测序的灵芝(G. lucidum)和中华灵芝(G. sinense)的线粒体DNA相比,三个有丝分裂基因组中蛋白质和rRNA基因的基因顺序高度保守;然而,tRNA组成略有不同。梅雷迪灵芝的线粒体基因组将有助于了解灵芝属和灵芝科的系统发育和进化,该类群包含许多具有高药用价值的物种。