Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark.
APMIS. 2022 Jul;130(7):359-370. doi: 10.1111/apm.13101. Epub 2021 Mar 1.
In this descriptive pilot study, we aim to establish a porcine Staphylococcus aureus skin infection model by subcutaneous injection (s.c.) of the porcine S54F9 S. aureus strain in the groin area. Six pigs were used in the study: Five pigs were injected with S. aureus, inocula ranging from 7 × 10 to 5 × 10 colony-forming units per kg bodyweight; one pig was injected with saline exclusively. Lesions were recorded up to 6 days postinoculation using clinical evaluation, ultrasound evaluation, microbiology, flow cytometry, and pathology. Inoculation gave rise to lesions ranging from localized skin infection, that is, minute histological changes, intracellular infection, and macroscopic abscess formation with sequestration of soft tissue, to generalized infection and development of disseminated intravascular coagulation necessitating euthanasia only 10 h after inoculation. Ultrasound assessment of maximum width and characteristics was not able to disclose the progress of the local infection. Flow cytometry and immunohistochemistry revealed the participation of γδT cells in the immune response. In conclusion, we did see a graded inflammatory response associated with the dose of s.c. inoculated bacteria, which may be useful for studying, in particular, the interaction of bacteria and inflammatory mononuclear cell populations. It needs to be investigated if the model is discriminatory and robust.
在这项描述性的初步研究中，我们旨在通过在腹股沟区域皮下注射猪 S54F9 金黄色葡萄球菌株来建立猪金黄色葡萄球菌皮肤感染模型。本研究使用了六头猪：五头猪被注射了金黄色葡萄球菌，接种物的浓度范围为每公斤体重 7×10 到 5×10 个菌落形成单位；一头猪仅注射盐水。在接种后 6 天内，通过临床评估、超声评估、微生物学、流式细胞术和病理学记录病变。接种导致病变范围从局部皮肤感染（即微小组织学变化）、细胞内感染和肉眼脓肿形成伴软组织隔离，到全身感染和弥散性血管内凝血发展，仅在接种后 10 小时就需要安乐死。超声评估最大宽度和特征不能揭示局部感染的进展。流式细胞术和免疫组织化学显示 γδT 细胞参与了免疫反应。总之，我们确实看到了与皮下接种细菌剂量相关的分级炎症反应，这可能对研究细菌和炎症单核细胞群的相互作用特别有用。需要研究该模型是否具有区分性和稳健性。
