DU Yu-Zhong, Su Jie, Yan Mei-Qiu, Chen Su-Hong, Lyu Gui-Yuan, Yu Jing-Jing
Zhejiang Chinese Medical University Hangzhou 310053, China.
Zhejiang University of Technology Hangzhou 310014, China.
Zhongguo Zhong Yao Za Zhi. 2021 Jan;46(1):190-195. doi: 10.19540/j.cnki.cjcmm.20200915.408.
The aim of this paper was to study the improvement effect of ethanol extract from Citri Reticulatae Pericarpium(CRP) on triglyceride of hyperlipidemia model rats, and to explore the possible mechanism. SD rats were randomly divided into normal group, model group, positive control group, and high, medium and low-dose CRP ethanol extract groups, with 10 rats in each group. During the experiment, except for the normal group that was fed with distilled water and ordinary feed, rats in the other groups were given different concentrations of alcohol and fed with high-sugar and fat diets. All rats were given free diets. While being modeled, each group was administered with 0.01 mL·g~(-1) by gavage once a day for six weeks. Blood samples were collected after two weeks, four weeks and six weeks of drug treatment. After the completion of the experiment, blood, liver and adipose tissue were collected. Triglyceride(TG), alanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP) in serum, TG in liver tissue and TG in fecal were detected. Free fatty acid(FFA) and triglyceride-related hydrolase, such as adipose tiglyceride lipase(ATGL), lipoprotein lipase(LPL), hepatic lipase(HL), hormone-sensitive triglyceride lipase(HSL) were detected by ELISA. The mRNA expressions of peroxisome proliferators-activated receptors(PPARγ), sterol regulatory element binding protein 1 c(SREBP-1 c) and farnesoid X receptor(FXR) were determined by RT-PCR. Compared with the model group, each administration group could reduce TG levels in serum and liver to varying degrees, reduce serum ALT, AST, ALP activities, significantly reduce free fatty acid content in serum, significantly increase triglyceride metabolism-related enzymes, including fat ATGL, LPL and liver HL content, and significantly reduced the content of fat HSL. According to the study of transcriptional regulation genes relating to triglyceride metabolism, extract from CRP could significantly increase the mRNA expressions of PPARγ and FXR. In conclusion, ethanol extract from CRP could ob-viously reduce the TG level of hyperlipidemia model rats, and might reduce plasma TG content by increasing PPARγ-LPL/ATGL and FXR-HL triglyceride hydrolysis pathways.
本文旨在研究陈皮乙醇提取物对高脂血症模型大鼠甘油三酯的改善作用，并探讨其可能机制。将SD大鼠随机分为正常组、模型组、阳性对照组以及陈皮乙醇提取物高、中、低剂量组，每组10只。实验期间，除正常组喂饲蒸馏水和普通饲料外，其他各组大鼠给予不同浓度酒精并喂饲高糖高脂饲料，所有大鼠自由饮食。造模同时，每组每天灌胃给药1次，剂量为0.01 mL·g-1，共6周。药物治疗2周、4周、6周后采集血样。实验结束后，采集血液、肝脏和脂肪组织。检测血清中甘油三酯（TG）、谷丙转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP），肝组织中TG以及粪便中TG。采用酶联免疫吸附测定法（ELISA）检测游离脂肪酸（FFA）以及甘油三酯相关水解酶，如脂肪甘油三酯脂肪酶（ATGL）、脂蛋白脂肪酶（LPL）、肝脂肪酶（HL）、激素敏感性甘油三酯脂肪酶（HSL）。采用逆转录聚合酶链反应（RT-PCR）检测过氧化物酶体增殖物激活受体（PPARγ）、固醇调节元件结合蛋白1c（SREBP-1c）和法尼酯X受体（FXR）的mRNA表达。与模型组相比，各给药组均可不同程度降低血清和肝脏中TG水平，降低血清ALT、AST、ALP活性，显著降低血清中游离脂肪酸含量，显著升高甘油三酯代谢相关酶，包括脂肪组织ATGL、LPL和肝脏HL含量，并显著降低脂肪组织HSL含量。通过对甘油三酯代谢相关转录调控基因的研究发现，陈皮提取物可显著升高PPARγ和FXR的mRNA表达。综上所述，陈皮乙醇提取物可明显降低高脂血症模型大鼠的TG水平，可能通过增加PPARγ-LPL/ATGL和FXR-HL甘油三酯水解途径降低血浆TG含量。
