高直链淀粉玉米中淀粉生物合成基因的表达模式与淀粉分子结构的关系
Expression Pattern of Starch Biosynthesis Genes in Relation to the Starch Molecular Structure in High-Amylose Maize.
作者信息
Zhong Yuyue, Qu Jianzhou, Blennow Andreas, Liu Xingxun, Guo Dongwei
机构信息
Key Laboratory of Biology and Genetic Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture, College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China.
Lab of Food Soft Matter Structure and Advanced Manufacturing, College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China.
出版信息
J Agric Food Chem. 2021 Mar 10;69(9):2805-2815. doi: 10.1021/acs.jafc.0c07354. Epub 2021 Mar 1.
The molecular structure and the expression levels of starch biosynthesis-related genes of three types of high-amylose maize (HAM) genotypes and one normal maize (NM) genotype at 5-35 days after pollination (DAP) were studied. Size exclusion chromatography (SEC) analysis showed that the molecular size of amylopectin molecules in NM increased from 5 to 35 DAP and the amylose content in HAM genotypes increased from 15 to 35 DAP. Correlation analysis for both NM and HAMs combined showed that and were negatively correlated with the contents of amylose and long amylopectin chains (DP > 30) and positively correlated with the content of short amylopectin chains (DP ≤ 31) and the molecular size of amylopectin molecules. Correlation analysis for only the HAMs showed that amylose content was negatively correlated with and . In both correlation analyses, showed a negative correlation with the average chain lengths of amylose chains.
研究了三种高直链淀粉玉米（HAM）基因型和一种普通玉米（NM）基因型在授粉后5 - 35天（DAP）淀粉生物合成相关基因的分子结构和表达水平。尺寸排阻色谱（SEC）分析表明，NM中支链淀粉分子的分子大小在授粉后5至35天增加，而HAM基因型中的直链淀粉含量在授粉后15至35天增加。对NM和HAM组合进行的相关性分析表明，[此处原文缺失相关基因信息]与直链淀粉和长支链淀粉链（DP > 30）的含量呈负相关，与短支链淀粉链（DP ≤ 31）的含量和支链淀粉分子的分子大小呈正相关。仅对HAM进行的相关性分析表明，直链淀粉含量与[此处原文缺失相关基因信息]呈负相关。在这两种相关性分析中，[此处原文缺失相关基因信息]与直链淀粉链的平均链长呈负相关。
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