Brunner G, Schirrmacher V
German Cancer Research Centre, Institute of Immunology and Genetics, Heidelberg, Federal Republic of Germany.
Anal Biochem. 1988 Feb 1;168(2):411-6. doi: 10.1016/0003-2697(88)90337-5.
An electrophoretic modification of the conventional fibrin autography that can be used for the detection of plasminogen activators (urokinase type and tissue type) and fibrin-degrading enzymes in complex biological fluids is described. After separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the proteins and the substrate plasminogen are transferred electrophoretically into the fibrin indicator gel, resulting in an efficient transfer of proteinases as well as high resolution and contrast of fibrinolytic zones caused by plasminogen activator activity. Picogram amounts of human urokinase type plasminogen activator (about 0.002 International Unit) are still detectable. The technique is also applicable to reversed fibrin autography for plasminogen activator inhibitors.
本文描述了一种传统纤维蛋白自显影的电泳改良方法，该方法可用于检测复杂生物流体中的纤溶酶原激活剂（尿激酶型和组织型）和纤维蛋白降解酶。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离后，蛋白质和底物纤溶酶原通过电泳转移到纤维蛋白指示凝胶中，从而实现蛋白酶的高效转移以及纤溶酶原激活剂活性引起的纤维蛋白溶解区的高分辨率和高对比度。皮克级量的人尿激酶型纤溶酶原激活剂（约0.002国际单位）仍可检测到。该技术也适用于纤溶酶原激活剂抑制剂的反向纤维蛋白自显影。
