Izumi T, Shibata Y, Yamamoto T
Department of Anatomy, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Anat Rec. 1988 Mar;220(3):225-32. doi: 10.1002/ar.1092200302.
The cytoplasmic surface of plasmalemmal vesicles in aortic endothelial cells was examined in quick-freeze, deep-etching replicas. In addition to the clathrin-coated vesicles, striped patterns were observed over the cytoplasmic surface membranes of small vesicles (60-80 nm in diameter) in the unfixed specimens. These patterns were more clearly visible in saponin-extracted specimens; the stripes were composed of several ridges or strands of 6-10 nm in width, and some were crossed. These were distinct from the characteristic pentagons or hexagons of the clathrin-coated vesicles. This striped structure was enhanced by treatment with myosin subfragment-1 or phalloidin, thereby indicating a possible relation to actin filaments. Patchy plaques with similar striped patterns appeared on the cytoplasmic surface of the plasmalemma proper and also on the cytoplasmic vacuole membranes together with clathrin. These striped structures may be involved in the formation and transport of so-called "uncoated" vesicles.
在快速冷冻、深度蚀刻复制品中检查了主动脉内皮细胞质膜小泡的胞质面。除了网格蛋白包被小泡外，在未固定标本中，直径60 - 80 nm的小泡的胞质表面膜上观察到条纹状图案。这些图案在皂角苷提取的标本中更清晰可见；条纹由几条宽度为6 - 10 nm的嵴或条带组成，有些相互交叉。这些与网格蛋白包被小泡的特征性五边形或六边形不同。用肌球蛋白亚片段-1或鬼笔环肽处理可增强这种条纹结构，从而表明其可能与肌动蛋白丝有关。具有相似条纹图案的斑块状斑出现在质膜本身的胞质面以及与网格蛋白一起的胞质液泡膜上。这些条纹结构可能参与所谓“无包被”小泡的形成和运输。
