College of Chemistry and Material Science, Food Safety Analysis and Test Engineering Technology Research Center of Shandong Province, Shandong Agricultural University, Taian 271018, PR China.
College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian 271018, PR China.
J Hazard Mater. 2021 Jul 15;414:125293. doi: 10.1016/j.jhazmat.2021.125293. Epub 2021 Feb 18.
A photoelectrochemical (PEC) biosensor was constructed for 5-formylcytosine (5fC) nucleotide detection based on AgS@WS photoactive material and FeVO catalytic signal quenching. After AgS@WS was modified onto the ITO substrate surface, 5fC recognition reagent of Au@4-amino3hydrazino5mercapto-1,2,4-triazol (Au@AHMT) was further modified through electrostatic adsorption. Afterwards, based on the specific chemical reaction between -NH and -CHO, 5fC can be selectively recognized and captured. Subsequently, the nanoenzyme of FeVO was recognized based on the specific reaction between the phosphate group of 5fC nucleotide and Fe. Under the catalysis of FeVO, the 4-chloro-1-naphthol in the detection solution can be oxidized to generate a precipitate, which will be retained on the electrode surface to inhibit the PEC signal. The developed method presented a widely dynamic range from 0.1 to 400 nM. The detection limit was 0.062 nM (3σ). This method also showed good detection selectivity, reproducibility and stability. The applicability was verified by investigating 5fC content change in genomic DNA of rice tissues after incubated with heavy metals. Moreover, the inhibited influence of hydrochar towards heavy metals was also assessed.
基于 AgS@WS 光活性材料和 FeVO 催化信号猝灭作用，构建了用于检测 5-甲酰胞嘧啶核苷（5fC）的光电化学（PEC）生物传感器。AgS@WS 修饰在 ITO 基底表面后，通过静电吸附进一步修饰 5fC 识别试剂金@4-氨基-3-肼基-5-巯基-1,2,4-三唑（Au@AHMT）。随后，基于-NH 和 -CHO 之间的特定化学反应，可以选择性地识别和捕获 5fC。随后，基于 5fC 核苷酸的磷酸基团与 Fe 的特异性反应，识别纳米酶 FeVO。在 FeVO 的催化作用下，检测溶液中的 4-氯-1-萘酚可以被氧化生成沉淀物，该沉淀物将保留在电极表面以抑制 PEC 信号。所开发的方法呈现出从 0.1 到 400 nM 的宽动态范围。检测限为 0.062 nM（3σ）。该方法还表现出良好的检测选择性、重现性和稳定性。通过研究重金属孵育后水稻组织基因组 DNA 中 5fC 含量的变化，验证了该方法的适用性。此外，还评估了水炭对重金属的抑制影响。
