Zhu Lu, Jing Jing, Qin Shuaiqi, Zheng Qi, Lu Jiani, Zhu Cuiyun, Liu Ya, Fang Fugui, Li Yunsheng, Ling Yinghui
College of Animal Science and Technology, Anhui Agricultural University, Anhui Hefei, 230036, China; Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Anhui Hefei, 230036, China.
College of Animal Science and Technology, Anhui Agricultural University, Anhui Hefei, 230036, China; Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Anhui Hefei, 230036, China.
Theriogenology. 2021 Apr 15;165:92-98. doi: 10.1016/j.theriogenology.2021.02.012. Epub 2021 Feb 19.
MicroRNAs (miRNAs) are key epigenomic regulators of proliferation, differentiation, and secretion in cells involved in follicular development. We here studied the functional role of one such molecule, miR-130a-3p, in goat ovarian granulosa cells (GCs). High expression of this miRNA was evident in goat GCs by fluorescence in situ hybridization and suppressed estradiol and progesterone secretion from these cells, as determined by ELISA. miR-130a-3p was predicted to have a binding site for the 3' UTR of the prostate transmembrane protein androgen induced 1 gene (PMEPA1), and this was verified by a dual-luciferase reporter assay. PMEPA1 mRNA and protein expression were both found to be regulated by miR-130a-3p in GCs. Moreover, the overexpression or knockdown of PMEPA1 enhanced or suppressed estradiol and progesterone secretion from these cells, respectively. Furthermore, the secretion of estradiol and progesterone did not change significantly after the offsetting of PMEPA1 overexpression in GCs by miR-130a-3p. In summary, our present data indicate that miR-130a-3p inhibits the secretion of estradiol and progesterone in GCs by targeting PMEPA1. Our study thus provides seminal data and important new insights into the regulation of reproductive mechanisms in the nanny goat and other female mammals.
微小RNA（miRNA）是参与卵泡发育的细胞增殖、分化和分泌的关键表观基因组调节因子。我们在此研究了一种此类分子miR-130a-3p在山羊卵巢颗粒细胞（GCs）中的功能作用。通过荧光原位杂交在山羊GCs中明显可见该miRNA的高表达，并且如通过酶联免疫吸附测定所确定的，其抑制了这些细胞中雌二醇和孕酮的分泌。预测miR-130a-3p在前列腺跨膜蛋白雄激素诱导1基因（PMEPA1）的3'非翻译区具有结合位点，并且通过双荧光素酶报告基因测定对其进行了验证。发现PMEPA1的mRNA和蛋白质表达在GCs中均受miR-130a-3p调控。此外，PMEPA1的过表达或敲低分别增强或抑制了这些细胞中雌二醇和孕酮的分泌。此外，在GCs中miR-130a-3p抵消PMEPA1过表达后，雌二醇和孕酮的分泌没有显著变化。总之，我们目前的数据表明miR-130a-3p通过靶向PMEPA1抑制GCs中雌二醇和孕酮的分泌。因此，我们的研究为奶山羊和其他雌性哺乳动物生殖机制的调控提供了开创性数据和重要的新见解。
