Mohammadzadeh Masoomeh, Pourentezari Majid, Zare-Zardini Hadi, Nabi Ali, Esmailabad Saeed Ghasemi, Khodadadian Ali, Talebi Ali Reza
Department of Reproductive Biology, Yazd Reproductive Sciences Institute, Research and Clinical Center for Infertility, Yazd, Iran.
Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Clin Exp Reprod Med. 2021 Mar;48(1):34-42. doi: 10.5653/cerm.2020.03524. Epub 2021 Feb 22.
Studies of the effects of estrogens on the male reproductive system have emphasized the role of these hormones in male fertility. Sesame oil has many phytoestrogenic compounds and may improve male fertility. This study investigated the effects of sesame oil and different concentrations of estrogen on sperm parameters and DNA integrity in male mice.
Twenty old NMRI (The Naval Medical Research Institute) male mice (40 weeks; weight, 30-35 g) were treated with sesame oil or different concentrations of estrogen (estradiol, 1 and 10 μL/kg/ day) or received no treatment (controls). After 35 days, sperm parameters and DNA integrity were assessed and analyzed.
Sperm count, progressive motility, and morphology were decreased in the group that received 10 μL/kg of estradiol. A remarkably lower percentage of DNA fragmentation and protamine deficiency were detected in the group that received 1 μL/kg of estradiol. In the groups that received sesame oil and 1 μL/kg of estradiol, the numbers of spermatogonia and Leydig cells were higher than in controls. The combination of sesame oil and 1 μL/kg of estradiol led to improved sperm parameters and chromatin and testicular structure.
Based on this study, consumption of sesame oil and a low concentration of estradiol may improve testicular function in older mice.
关于雌激素对雄性生殖系统影响的研究着重强调了这些激素在男性生育能力方面的作用。芝麻油含有多种植物雌激素化合物，可能改善男性生育能力。本研究调查了芝麻油和不同浓度雌激素对雄性小鼠精子参数及DNA完整性的影响。
将20只老龄NMRI（海军医学研究所）雄性小鼠（40周龄；体重30 - 35克）分别用芝麻油、不同浓度的雌激素（雌二醇，1和10微升/千克/天）处理，或不进行处理（作为对照）。35天后，评估并分析精子参数和DNA完整性。
接受10微升/千克雌二醇处理的组中，精子计数、前向运动能力和形态均下降。接受1微升/千克雌二醇处理的组中，检测到DNA片段化和鱼精蛋白缺乏的比例显著降低。在接受芝麻油和1微升/千克雌二醇处理的组中，精原细胞和睾丸间质细胞的数量高于对照组。芝麻油与1微升/千克雌二醇的组合使精子参数、染色质和睾丸结构得到改善。
基于本研究，食用芝麻油和低浓度雌二醇可能改善老龄小鼠的睾丸功能。
