Department of Respiratory & Allergy, Third Affiliated Hospital of Shenzhen University, Shenzhen 518020, China; School of Public Health, Health Science Center, Shenzhen University, Shenzhen, Guangdong Province 518060, China.
Qingdao Municipal Center for Disease Control and Prevention, 175 Shandong Road, Shibei District, Qingdao, Shandong Province 266033, China.
Food Res Int. 2021 Feb;140:109997. doi: 10.1016/j.foodres.2020.109997. Epub 2020 Dec 15.
Here, the potential allergenicity of shrimp tropomyosin (TM) after conjugation with chlorogenic acid (CA) and (-)-epigallo-catechin 3-gallate (EGCG) was assessed. Conformational structures of TM-polyphenol complexes were detected using SDS-PAGE, circular dichroism (CD), and fluorescence. Potential allergenicity was assessed by immunological methods, a rat basophil leukemia cell model (RBL-2H3), and in vivo assays. Indirect ELISA showed that TM-polyphenol complexes caused a conformational change to TM structure, with decreased IgG/IgE binding capacity significantly fewer inflammatory mediators were released with EGCG-TM and CA-TM in a mediator-releasing RBL-2H3 cell line. Mice model showed low allergenicity to serum levels of TM-specific antibody and T-cell cytokine production. EGCG-TM and CA-TM might reduce the potential allergenicity of shrimp TM, which could be used to produce hypoallergenic food in the food industry.
在这里，评估了虾肌球蛋白（TM）与绿原酸（CA）和（-）-表没食子儿茶素 3-没食子酸酯（EGCG）缀合后的潜在致敏性。使用 SDS-PAGE、圆二色性（CD）和荧光检测 TM-多酚复合物的构象结构。通过免疫学方法、大鼠嗜碱性白血病细胞模型（RBL-2H3）和体内试验评估潜在的致敏性。间接 ELISA 表明，TM-多酚复合物导致 TM 结构发生构象变化，与 EGCG-TM 和 CA-TM 相比，IgG/IgE 结合能力显著降低，释放的炎症介质也明显减少在释放介质的 RBL-2H3 细胞系中。小鼠模型显示 TM 特异性抗体的血清水平和 T 细胞细胞因子产生的低致敏性。EGCG-TM 和 CA-TM 可能降低虾 TM 的潜在致敏性，可用于食品工业生产低致敏性食品。
