Ersoy Selvi C, Chambers Henry F, Proctor Richard A, Rosato Adriana E, Mishra Nagendra N, Xiong Yan Q, Bayer Arnold S
The Lundquist Institute, Torrance, CA, USA.
UCSF School of Medicine, San Francisco, CA, USA.
Antimicrob Agents Chemother. 2023 May 1;65(5). doi: 10.1128/AAC.02621-20. Epub 2021 Mar 1.
Certain methicillin-resistant (MRSA) strains exhibit β-lactam-susceptibility , and in the presence of NaHCO (NaHCO-responsive MRSA). Herein, we investigate the impact of NaHCO on factors required for PBP2a functionality. Prototype NaHCO-responsive and -nonresponsive MRSA strains (as defined ) were assessed for the impact of NaHCO on: expression of genes involved in PBP2a production-maturation pathways (, , , , , , and ); membrane PBP2a and PrsA protein content; and membrane carotenoid content. Following NaHCO exposure in NaHCO-responsive (vs - nonresponsive) MRSA, there was significantly reduced expression of: ) and ; ) the gene axis; and ) Carotenoid production was reduced, while expression was increased by NaHCO exposure in all MRSA strains. This work underscores the distinct regulatory impact of NaHCO on a cadre of genes encoding factors required for maintenance of the MRSA phenotype through PBP2a functionality and maturation.
某些耐甲氧西林金黄色葡萄球菌（MRSA）菌株表现出β-内酰胺敏感性，且在有NaHCO（NaHCO反应型MRSA）存在的情况下。在此，我们研究了NaHCO对PBP2a功能所需因子的影响。评估了典型的NaHCO反应型和非反应型MRSA菌株（如所定义），以研究NaHCO对以下方面的影响：参与PBP2a产生-成熟途径的基因（、、、、、和）的表达；膜PBP2a和PrsA蛋白含量；以及膜类胡萝卜素含量。在NaHCO反应型（与非反应型）MRSA中暴露于NaHCO后，以下基因的表达显著降低：）和；）基因轴；以及）。类胡萝卜素产量降低，而在所有MRSA菌株中，NaHCO暴露使表达增加。这项工作强调了NaHCO对一系列通过PBP2a功能和成熟维持MRSA表型所需因子的编码基因具有独特的调控影响。
