苯妥英钠与地塞米松在培养的牙髓干细胞中成骨潜能的比较
Comparison of Osteogenic Potential of Phenytoin with Dexamethasone in Cultured Dental Pulp Stem Cells.
作者信息
Asgharian-Rezaee Mitra, Alipour-Farmad Raheleh, Tayarani-Najaran Zahra
机构信息
Department of Toxicology and Pharmacology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
出版信息
Rep Biochem Mol Biol. 2020 Oct;9(3):331-337. doi: 10.29252/rbmb.9.3.331.
BACKGROUND
One of the adverse effects of phenytoin (diphenylhydantoin, DPH) is enlargement of facial features. Although there are some reports on anabolic action of phenytoin on bone cells, the osteogenic potential of DPH on mesenchymal stem cells has not been studied. The purpose of this study was to evaluate the osteogenic potential of DPH on dental pulp stem cells (DPSCs).
METHODS
Human DPSCs were isolated and characterized by flow cytometry; presence of CD29 and CD44 and absence of CD34 and CD45 were performed to confirm the mesenchymal stem cells. Isolated DPSCs were differentiated either in conventional osteogenic medium with Dexamethasone or medium containing different concentration of phenytoin (12.5, 25, 100, and 200 µM). The osteogenic differentiation evaluated by performing western blot test for Runt-related transcription factor 2 (RUNX2), osteopontin and alkaline phosphatase (ALP) also alizarin red S staining to measure the mineralization of cells.
RESULTS
Our results showed morphological changes and mineralization of DPSCs by using DPH were comparable with dexamethasone. Moreover, western blot results of DPH group showed significant increase of ALP, RUNX2 and osteopontin (OSP) in comparison with control.
CONCLUSION
The data of present study showed the osteogenic activity of phenytoin, considering as an alternative of dexamethasone for inducing osteogenic differentiation of dental pulp stem cells.
背景
苯妥英钠（二苯乙内酰脲，DPH）的不良反应之一是面部特征增大。尽管有一些关于苯妥英钠对骨细胞合成代谢作用的报道，但尚未研究DPH对间充质干细胞的成骨潜能。本研究的目的是评估DPH对牙髓干细胞（DPSCs）的成骨潜能。
方法
分离人DPSCs并通过流式细胞术进行鉴定；检测CD29和CD44的存在以及CD34和CD45的缺失以确认间充质干细胞。将分离的DPSCs在含有地塞米松的传统成骨培养基或含有不同浓度苯妥英钠（12.5、25、100和200μM）的培养基中进行分化。通过对 runt相关转录因子2（RUNX2）、骨桥蛋白和碱性磷酸酶（ALP）进行蛋白质印迹试验以及茜素红S染色来评估成骨分化，以测量细胞的矿化情况。
结果
我们的结果表明，使用DPH时DPSCs的形态变化和矿化与地塞米松相当。此外，与对照组相比，DPH组的蛋白质印迹结果显示ALP、RUNX2和骨桥蛋白（OSP）显著增加。
结论
本研究数据显示了苯妥英钠的成骨活性，可将其视为诱导牙髓干细胞成骨分化的地塞米松替代品。
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