Khanam Jyosna, Hossain Delowar, Hosen Bayejid, Uddin Mesbah, Kabir Asadul, Anwarul Bari Mohammad
Institute of Nutrition and Food Science, University of Dhaka, Bangladesh.
Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh.
Rep Biochem Mol Biol. 2020 Oct;9(3):366-372. doi: 10.29252/rbmb.9.3.366.
Myocardial infarction is one of the leading causes of morbidity and mortality worldwide. Oxidative stress plays a vital role in the pathogenesis of atherosclerosis leading to myocardial infarction and Glutathione S-transferases (GSTs) act as detoxifying enzymes to reduce oxidative stress. The aim of the present study was to investigate the associations of the GST (T1 & M1) gene polymorphism with the susceptibility of myocardial infarction in the Bangladeshi population.
A case-control study on 100 cardiac patients with MI and 150 control subjects was conducted. The genotyping of GST (T1 & M1) gene was done using conventional Polymerase Chain Reaction.
The percentage of GSTM1 genotypes was significantly (p< 0.01) lower in patients compared to control subjects while the GSTT1 genotypes were not significantly different between the study subjects. The individual with GSTM1 null allele was at 2.5-fold increased risk {odds ratio (OR)= 2.5; 95 % confidence interval (95 % CI)= 1.4 to 4.3; p< 0.01} of experiencing MI while individual with either GSTM1 or GSTT1 genotypes was at lower risk. In the case of GST M1 and GST T1 combined genotype, patients having both null genotypes for GST M1 and GST T1 gene showed significantly (p< 0.01) higher risk of experiencing MI when compared to control subjects (OR= 3.5; 95% CI= 1.7-7.2; p< 0.001).
Thus our recent study suggested that GSTM1 alone and GSTM1 and T1 in combination augments the risk of MI in Bangladeshi population.
心肌梗死是全球发病和死亡的主要原因之一。氧化应激在导致心肌梗死的动脉粥样硬化发病机制中起关键作用，而谷胱甘肽S - 转移酶（GSTs）作为解毒酶可减轻氧化应激。本研究的目的是调查GST（T1和M1）基因多态性与孟加拉人群中心肌梗死易感性的关联。
对100例心肌梗死心脏病患者和150例对照者进行了病例对照研究。使用常规聚合酶链反应对GST（T1和M1）基因进行基因分型。
与对照者相比，患者中GSTM1基因型的百分比显著降低（p < 0.01），而GSTT1基因型在研究对象之间无显著差异。携带GSTM1无效等位基因的个体发生心肌梗死的风险增加2.5倍{比值比（OR）= 2.5；95%置信区间（95%CI）= 1.4至4.3；p < 0.01}，而携带GSTM1或GSTT1基因型的个体风险较低。在GST M1和GST T1联合基因型的情况下，与对照者相比，同时具有GST M1和GST T1基因无效基因型的患者发生心肌梗死的风险显著更高（p < 0.01）（OR = 3.5；95%CI = 1.7 - 7.2；p < 0.001）。
因此，我们最近的研究表明，单独的GSTM1以及GSTM1和T1联合增加了孟加拉人群发生心肌梗死的风险。
