双歧杆菌转化。

Bifidobacterium Transformation.

机构信息

APC Microbiome Ireland, University College Cork, Cork, Ireland.

Danone Nutricia Research, Utrecht, The Netherlands.

出版信息

Methods Mol Biol. 2021;2278:13-19. doi: 10.1007/978-1-0716-1274-3_2.

DOI:10.1007/978-1-0716-1274-3_2

PMID:33649944

Abstract

The protocol presented in this chapter describes a generic method for electrotransformation of Bifidobacterium spp., outlining a technique that is ideal for conferring selective properties onto strains as well as allowing the user to introduce or knock out/in selected genes for phenotypic characterization purposes. We have generalized on the plasmid chosen for transformation and antibiotic selection marker, but the protocol is versatile in this respect and we are able to achieve transformation efficiencies up to 10 transformants/μg of DNA.

摘要

本章提出的方案描述了双歧杆菌属电转化的通用方法，概述了一种将选择性特性赋予菌株的理想技术，同时允许用户为表型特征目的引入或敲除/敲入选定基因。我们已经对转化和抗生素选择标记所选择的质粒进行了概括，但该方案在这方面具有通用性，我们能够实现高达 10 个转化体/μg DNA 的转化效率。