Sichuan Animal Science Academy, Chengdu, Sichuan 610066 China; Animal Breeding and Genetics Key Laboratory of Sichuan Province, Chengdu, Sichuan 610066 China.
Sichuan Animal Science Academy, Chengdu, Sichuan 610066 China; Animal Breeding and Genetics Key Laboratory of Sichuan Province, Chengdu, Sichuan 610066 China.
Poult Sci. 2021 Mar;100(3):100932. doi: 10.1016/j.psj.2020.12.029. Epub 2021 Jan 23.
As a ubiquitous heavy metal, cadmium (Cd) is highly toxic to various organs. However, the effects and molecular mechanism of Cd toxicity in the chicken heart remain largely unknown. The goal of our study was to investigate the cardiac injury in chickens' exposure to Cd. We detected the levels of oxidative stress-related molecules in the Cd-induced chicken heart, and assessed the histopathological changes by hematoxylin and eosin staining. RNA sequencing was performed to identify differentially expressed mRNAs between the Cd-induced group and control group. The expression of candidate genes involved in oxidative stress was certified by quantitative reverse transcription PCR. Our results showed that the expression of glutathione, peroxidase, and superoxide dismutase was significantly decreased and malondialdehyde was increased in the heart of chickens by Cd induction. The disorderly arranged cardiomyocytes, swelled and enlarged cells, partial cardiomyocyte necrosis, blurred morphological structure, and notable inflammatory cell infiltration were observed in the Cd-induced chicken heart. RNA sequencing identified 23 upregulated and 11 downregulated mRNAs in the heart tissues of the chicken in the Cd-induced group, and functional pathways indicated that they were associated with oxidative stress. Moreover, CREM, DUSP8, and ITGA11 expressions were significantly reduced, whereas LAMA1 expression was induced in heart tissue of chickens by Cd treatment. Overall, our findings revealed that oxidative stress and pathological changes in the chicken heart could be triggered by Cd. The mRNA transcriptional profiles identified differentially expressed genes in the chicken heart by Cd induction, revealing oxidative stress-related key genes and enhancing our understanding of Cd toxicity in the chicken heart.
作为一种普遍存在的重金属，镉（Cd）对各种器官都有很高的毒性。然而，镉对鸡心脏的毒性作用及其分子机制在很大程度上仍不清楚。本研究旨在探讨镉暴露对鸡心脏的损伤作用。我们检测了镉诱导的鸡心脏中氧化应激相关分子的水平，并通过苏木精-伊红染色评估了组织病理学变化。进行 RNA 测序以鉴定 Cd 诱导组和对照组之间差异表达的 mRNAs。通过定量逆转录 PCR 验证候选基因参与氧化应激的表达。研究结果表明，镉诱导导致鸡心脏中谷胱甘肽、过氧化物酶和超氧化物歧化酶的表达显著降低，而丙二醛的含量增加。镉诱导的鸡心脏中观察到排列紊乱的心肌细胞、肿胀和增大的细胞、部分心肌细胞坏死、形态结构模糊以及明显的炎性细胞浸润。RNA 测序鉴定出 Cd 诱导组鸡心脏组织中 23 个上调和 11 个下调的 mRNAs，功能途径表明它们与氧化应激有关。此外，CREM、DUSP8 和 ITGA11 的表达显著降低，而 LAMA1 的表达在镉处理的鸡心脏组织中被诱导。综上所述，研究结果揭示了镉可能引发鸡心脏的氧化应激和病理变化。Cd 诱导的鸡心脏中差异表达基因的 mRNA 转录谱揭示了氧化应激相关的关键基因，增强了我们对镉对鸡心脏毒性的理解。
