Rácz Anita, Allan Brooke, Dwyer Toni, Thambithurai Davide, Crespel Amélie, Killen Shaun S
Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, Graham Kerr Building, University of Glasgow, Glasgow G12 8QQ, UK.
Department of Genetics, ELTE Eötvös Loránd University, Pázmány P.s. 1/C, H-1117 Budapest, Hungary.
Animals (Basel). 2021 Feb 26;11(3):616. doi: 10.3390/ani11030616.
In aquatic ecology, studies have commonly employed a tagging technique known as visible implant elastomer (VIE). This method has not been widely adopted by the zebrafish research community and also lacks refinement with regard to animal welfare. The current paper introduces a new VIE tagging protocol, with the aim of improving existing tagging techniques by placing particular emphasis on the Three Rs. To improve animal welfare and fish survival, we added the use of an analgesic compound (lidocaine) through the marking procedure, followed by after-treatment with antiseptics (melaleuca, aloe vera, and PVP-I as active ingredients) to improve tissue regeneration and healing. The newly improved protocol has been quantitatively evaluated on different populations and age groups of zebrafish. This study will be useful to the scientific zebrafish community and to the wider field including biologist and aquarists, especially in consideration of animal welfare, where tagging techniques are considered as a potential noxious stimulus for fish.
在水生生态学中,研究通常采用一种称为可视植入弹性体(VIE)的标记技术。这种方法尚未被斑马鱼研究界广泛采用,而且在动物福利方面也不够完善。本文介绍了一种新的VIE标记方案,旨在通过特别强调“3R”原则来改进现有的标记技术。为了提高动物福利和鱼类存活率,我们在标记过程中添加了一种镇痛化合物(利多卡因),随后用防腐剂(以白千层、芦荟和聚乙烯吡咯烷酮碘为活性成分)进行后续处理,以促进组织再生和愈合。新改进的方案已在不同种群和年龄组的斑马鱼上进行了定量评估。这项研究将对斑马鱼科学界以及包括生物学家和水族爱好者在内的更广泛领域有用,特别是考虑到动物福利,在这种情况下标记技术被视为对鱼类的潜在有害刺激。