Song Yu, Zoong Lwe Zolian S, Wickramasinghe Pallikonda Arachchige Dona Bashanee Vinusha, Welti Ruth
Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS 66506, USA.
Kansas Lipidomics Research Center, Kansas State University, Manhattan, KS 66506, USA.
Molecules. 2021 Feb 26;26(5):1273. doi: 10.3390/molecules26051273.
Head group-acylated chloroplast lipids were discovered in the 1960s, but interest was renewed about 15 years ago with the discovery of Arabidopsides E and G, acylated monogalactosyldiacylglycerols with oxidized fatty acyl chains originally identified in . Since then, plant biologists have applied the power of mass spectrometry to identify additional oxidized and non-oxidized chloroplast lipids and quantify their levels in response to biotic and abiotic stresses. The enzyme responsible for the head-group acylation of chloroplast lipids was identified as a cytosolic protein closely associated with the chloroplast outer membrane and christened acylated galactolipid-associated phospholipase 1 (AGAP1). Despite many advances, critical questions remain about the biological functions of AGAP1 and its head group-acylated products.
头部基团酰化的叶绿体脂质于20世纪60年代被发现，但大约15年前随着拟南芥糖苷E和G的发现，人们对其兴趣再次燃起，拟南芥糖苷E和G是酰化单半乳糖基二酰基甘油，其脂肪酸酰基链被氧化，最初在……中被鉴定出来。从那时起，植物生物学家利用质谱技术鉴定了更多氧化和未氧化的叶绿体脂质，并量化了它们在生物和非生物胁迫下的水平。负责叶绿体脂质头部基团酰化的酶被鉴定为一种与叶绿体外膜紧密相关的胞质蛋白，并被命名为酰化半乳糖脂相关磷脂酶1（AGAP1）。尽管取得了许多进展，但关于AGAP1及其头部基团酰化产物的生物学功能仍存在关键问题。
