Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynska Dolina F1, 842 48 Bratislava, Slovakia.
Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA.
Biosensors (Basel). 2021 Feb 26;11(3):63. doi: 10.3390/bios11030063.
Chymotrypsin is an important proteolytic enzyme in the human digestive system that cleaves milk proteins through the hydrolysis reaction, making it an interesting subject to study the activity of milk proteases. In this work, we compared detection of chymotrypsin by spectrophotometric dynamic light scattering (DLS) and quartz crystal microbalance (QCM) methods and determined the limit of chymotrypsin detection (LOD), 0.15 ± 0.01 nM for spectrophotometric, 0.67 ± 0.05 nM for DLS and 1.40 ± 0.30 nM for QCM methods, respectively. The sensors are relatively cheap and are able to detect chymotrypsin in 3035 min. While the optical detection methods are simple to implement, the QCM method is more robust for sample preparation, and allows detection of chymotrypsin in non-transparent samples. We give an overview on methods and instruments for detection of chymotrypsin and other milk proteases.
糜蛋白酶是人体消化系统中的一种重要的蛋白水解酶，通过水解反应分解牛奶蛋白，因此研究牛奶蛋白酶的活性是一个很有趣的课题。在这项工作中，我们比较了分光光度动态光散射（DLS）和石英晶体微天平（QCM）方法检测糜蛋白酶的效果，并分别确定了分光光度法、DLS 法和 QCM 法检测糜蛋白酶的下限（LOD），分别为 0.15±0.01 nM、0.67±0.05 nM 和 1.40±0.30 nM。这些传感器相对便宜，能够在 30-35 分钟内检测到糜蛋白酶。虽然光学检测方法易于实施，但 QCM 方法在样品制备方面更稳健，并且可以检测非透明样品中的糜蛋白酶。我们概述了检测糜蛋白酶和其他牛奶蛋白酶的方法和仪器。
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