Keqi Hu, Handong Liu
Department of Neurosurgery, Xiangyang Center Hospital, Affiliated Hospital of Hubei University of Arts and Science, Jingzhou Street 39, Xiangyang 441021, China.
Curr Cancer Drug Targets. 2021;21(6):526-535. doi: 10.2174/1568009621666210302090309.
The role and mechanism of long non-coding RNA cytoskeleton regulator (CYTOR) in Invasive Pituitary Adenomas (IPA) have not been elucidated previously.
This study aimed to investigate the interaction between CYTOR and miR-206 and their roles in IPA using HP75 cells as the model.
The expression levels of CYTOR and miR-206 were detected by quantitative real-time polymerase chain reaction (qRT-PCR) in IPA tissues and cell lines. The Chi-square test was used to analyze the correlation between CYTOR expression and clinical-pathological parameters. HP75 cell proliferation was detected by Cell Counting Kit-8 assay and colony formation assay. Scratch healing experiments and Transwell assay were used to detect migration and invasion of HP75 cells. The relationship between CYTOR and miR-206 was predicted by bioinformatics and verified by qRT-PCR and the dual-luciferase reporter gene method.
CYTOR is up-regulated in IPA tissues and cell lines. The high expression of CYTOR is associated with adenoma invasiveness and adenoma size of the patients. Down-regulation of CYTOR decreases the proliferation, migration and invasion of HP75 cells, while up-regulation of miR-206 can inhibit proliferation, migration and invasion of HP75 cells. MiR-206 is identified as a target of CYTOR and could be negatively regulated by it in IPA.
CYTOR, as a tumor-promoting factor, facilitates the proliferation, migration and invasion of HP75 cells through sponging miR-206.
The CYTOR-miR-206 axis provides new insights into the diagnosis and treatment of IPA.
长链非编码RNA细胞骨架调节因子（CYTOR）在侵袭性垂体腺瘤（IPA）中的作用和机制此前尚未阐明。
本研究旨在以HP75细胞为模型，探讨CYTOR与miR-206之间的相互作用及其在IPA中的作用。
采用定量实时聚合酶链反应（qRT-PCR）检测IPA组织和细胞系中CYTOR和miR-206的表达水平。采用卡方检验分析CYTOR表达与临床病理参数之间的相关性。采用细胞计数试剂盒-8法和集落形成试验检测HP75细胞增殖。采用划痕愈合实验和Transwell试验检测HP75细胞的迁移和侵袭能力。通过生物信息学预测CYTOR与miR-206之间的关系，并通过qRT-PCR和双荧光素酶报告基因法进行验证。
CYTOR在IPA组织和细胞系中上调。CYTOR的高表达与患者腺瘤的侵袭性和腺瘤大小有关。下调CYTOR可降低HP75细胞的增殖、迁移和侵袭能力，而上调miR-206可抑制HP75细胞的增殖、迁移和侵袭能力。miR-206被确定为CYTOR的靶点，在IPA中可被CYTOR负调控。
CYTOR作为一种肿瘤促进因子，通过吸附miR-206促进HP75细胞的增殖、迁移和侵袭。
CYTOR-miR-206轴为IPA的诊断和治疗提供了新的见解。
