College of Veterinary Medicine, Kangwon National University, Chuncheon24341, Korea.
Institute of Veterinary Science, Kangwon National University, Chuncheon24341, Korea.
Zygote. 2021 Aug;29(4):293-300. doi: 10.1017/S0967199420000891. Epub 2021 Mar 3.
This study was conducted to examine whether glucose in maturation medium containing reduced NaCl could improve oocyte maturation and embryonic development in pigs. The base medium was bovine serum albumin-free porcine zygote medium (PZM)-3 containing 10% (v/v) pig follicular fluid (FPZM) or 0.1% (w/v) polyvinyl alcohol (PPZM). Using each medium, the effects of NaCl concentrations (108 and 61.6 mM) and 5.56 mM glucose supplementation (designated as PZM108N, PZM108G, PZM61N, and PZM61G, respectively) were examined using a 2 × 2 factorial arrangement. When oocytes were matured in FPZM, glucose supplementation improved nuclear maturation compared with no supplementation, regardless of the NaCl concentrations. FPZM61G showed a higher blastocyst formation compared with FPZM108N and FPZM108G after parthenogenesis (PA). Blastocyst formations of somatic cell nuclear transfer (SCNT) embryos derived from FPZM61N and FPZM61G were higher compared with those of oocytes from FPZM108N. When oocytes were matured in PPZM, glucose added to PPZM108 and PPZM61 increased nuclear maturation compared with no supplementation. However, glucose added to PPZM108 did not alter embryonic development after PA. Additionally, oocytes matured in PPZM61G showed a higher blastocyst formation compared with those from PPZM61N. In SCNT, blastocyst formation was not influenced by glucose supplementation of PPZM108, but was increased by maturation in glucose-supplemented PPZM61. In embryonic development of in vitro fertilization (IVF), oocytes matured in medium with reduced NaCl and glucose showed significantly higher blastocyst formation compared with those matured in PPZM108G. Our results demonstrated that glucose in maturation medium containing 61.6 mM NaCl increased oocyte maturation and embryonic development after PA, SCNT, and IVF.
本研究旨在探讨成熟培养基中低浓度 NaCl 与葡萄糖共同作用是否可以提高猪卵母细胞的成熟和胚胎发育。基础培养基为无牛血清白蛋白的猪合子培养基（PZM）-3，内含 10%（v/v）猪卵泡液（FPZM）或 0.1%（w/v）聚乙烯醇（PPZM）。使用每种培养基，通过 2×2 析因设计，研究了 NaCl 浓度（108 和 61.6 mM）和 5.56 mM 葡萄糖添加（分别命名为 PZM108N、PZM108G、PZM61N 和 PZM61G）的影响。当卵母细胞在 FPZM 中成熟时，葡萄糖添加无论 NaCl 浓度如何均能提高核成熟率。与 FPZM108N 和 FPZM108G 相比，FPZM61G 在孤雌激活（PA）后具有更高的囊胚形成率。与来自 FPZM108N 的卵母细胞相比，来自 FPZM61N 和 FPZM61G 的体细胞核移植（SCNT）胚胎的囊胚形成率更高。当卵母细胞在 PPZM 中成熟时，与无添加相比，添加到 PPZM108 和 PPZM61 的葡萄糖均增加了核成熟率。然而，添加到 PPZM108 的葡萄糖并未改变 PA 后的胚胎发育。此外，在 PPZM61G 中成熟的卵母细胞具有更高的囊胚形成率，高于来自 PPZM61N 的卵母细胞。在 SCNT 中，葡萄糖添加到 PPZM108 不影响囊胚形成率，但葡萄糖添加到成熟的 PPZM61 则增加了囊胚形成率。在体外受精（IVF）的胚胎发育中，与在 PPZM108G 中成熟的卵母细胞相比，在含有低 NaCl 和葡萄糖的成熟培养基中成熟的卵母细胞具有显著更高的囊胚形成率。我们的结果表明，在含有 61.6 mM NaCl 的成熟培养基中添加葡萄糖可提高 PA、SCNT 和 IVF 后的卵母细胞成熟和胚胎发育。
