Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Kuantan 26300, Malaysia.
J Nanosci Nanotechnol. 2021 May 1;21(5):3059-3064. doi: 10.1166/jnn.2021.19283.
The fabrication of a network of glucose oxidase-horseradish peroxidase/tin oxide (GOx-HRP/SnO₂), immobilized onto a glassy carbon electrode (GCE) and its utilization as a biosensor for glucose detection is reported. The network established with GOx-HRP/SnO₂ possess high sensitivity and stability by performing the electrocatalytic features in the sensing of glucose. The turbidity of fabrication had been scanned and analyzed using UV-vis spectroscopy. The morphology and composition of the fabricated GOx-HRP/SnO₂ networks were characterized by scanning electron microscopy (SEM). Cyclic voltammetry and amperometry were employed to study the electrochemical properties of the proposed biosensor. The effect of applied electrode potential and pH were systemically investigated. The biosensor responds to glucose at work potential values between -400 mV, and exhibited a lower detection limit (0.025 mM) and long linear range (0.25 to 7.0 mM), and was resistant to common interferences.
报道了一种将葡萄糖氧化酶-辣根过氧化物酶/氧化锡（GOx-HRP/SnO₂）固定在玻碳电极（GCE）上的网络的制备及其作为葡萄糖检测生物传感器的应用。通过在葡萄糖检测中表现出的电催化特性，GOx-HRP/SnO₂ 建立的网络具有高灵敏度和稳定性。使用紫外可见光谱法扫描和分析了制造过程中的浊度。通过扫描电子显微镜（SEM）对制备的 GOx-HRP/SnO₂ 网络的形貌和组成进行了表征。采用循环伏安法和安培法研究了所提出的生物传感器的电化学性质。系统研究了施加的电极电势和 pH 值的影响。该生物传感器在-400 mV 的工作电位值下对葡萄糖有响应，并表现出较低的检测限（0.025 mM）和较长的线性范围（0.25 至 7.0 mM），并且对常见的干扰物质具有抗干扰性。
