Department of Pathology and Laboratory Medicine, University of Texas McGovern Medical School at Houston and Clinical Laboratories, Memorial-Hermann Hospital at Texas Medical Center, Houston, TX, USA
Department of Pathology and Laboratory Medicine, University of Texas McGovern Medical School at Houston and Clinical Laboratories, Memorial-Hermann Hospital at Texas Medical Center, Houston, TX, USA.
Ann Clin Lab Sci. 2021 Jan;51(1):102-105.
Biotin interferes with biotinylated antibody based immunoassays. We investigated effect of biotin on conventional troponin I and two high sensitivity troponin I assays, all manufactured by Siemens.
One high sensitivity troponin I assay (TNIH-1) was run using ADVIA Centaur analyzer. The second high sensitivity troponin I assay (TNIH-2) as well as conventional troponin I assay (CTNI) were run using Dimension Vista 1500 analyzer. We analyzed 25 specimens using CTNI, TNIH-1 and TNIH-2 assays for comparison of these assays. Moreover, serum pools prepared from additional specimens containing various amounts of troponin I were further supplemented with biotin to achieve biotin concentrations between 50 and 1000 ng/mL followed by reanalysis using CTNI, TNIH-1 and TNIH-2 assays.
Although both high sensitivity troponin I assays correlated well, there was a significant positive bias with TNIH-2. We observed no significant negative biotin interference at a level up to 250 ng/mL. Highest observed negative bias was 29.7%.
All three troponin I assays were free from biotin interferences up to a biotin concentration of 250 ng/mL.
生物素会干扰基于生物素化抗体的免疫测定。我们研究了生物素对西门子公司生产的常规肌钙蛋白 I 和两种高敏肌钙蛋白 I 检测试剂盒的影响。
使用 ADVIA Centaur 分析仪运行一种高敏肌钙蛋白 I 检测试剂盒（TNIH-1）。使用 Dimension Vista 1500 分析仪运行第二种高敏肌钙蛋白 I 检测试剂盒（TNIH-2）和常规肌钙蛋白 I 检测试剂盒（CTNI）。我们使用 CTNI、TNIH-1 和 TNIH-2 检测试剂盒分析了 25 个标本，以比较这些检测试剂盒。此外，我们从含有不同浓度肌钙蛋白 I 的额外标本中制备血清池，然后用生物素将其补充至 50 至 1000ng/mL 的浓度，再使用 CTNI、TNIH-1 和 TNIH-2 检测试剂盒进行重新分析。
尽管两种高敏肌钙蛋白 I 检测试剂盒相关性良好，但 TNIH-2 存在显著的正偏倚。我们在 250ng/mL 以下的浓度范围内没有观察到显著的负性生物素干扰。观察到的最大负性偏差为 29.7%。
在生物素浓度高达 250ng/mL 时，三种肌钙蛋白 I 检测试剂盒均不受生物素干扰。
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