生物素对 Vista 1500 和 ADVIA Centaur 分析仪中心肌钙蛋白 I 和高敏心肌肌钙蛋白 I 检测的影响。

Effect of Biotin on Cardiac Troponin I and High Sensitivity Cardiac Troponin I Assays on Vista 1500 and ADVIA Centaur Analyzer.

机构信息

Department of Pathology and Laboratory Medicine, University of Texas McGovern Medical School at Houston and Clinical Laboratories, Memorial-Hermann Hospital at Texas Medical Center, Houston, TX, USA

Department of Pathology and Laboratory Medicine, University of Texas McGovern Medical School at Houston and Clinical Laboratories, Memorial-Hermann Hospital at Texas Medical Center, Houston, TX, USA.

出版信息

Ann Clin Lab Sci. 2021 Jan;51(1):102-105.

PMID:33653787

Abstract

OBJECTIVE

Biotin interferes with biotinylated antibody based immunoassays. We investigated effect of biotin on conventional troponin I and two high sensitivity troponin I assays, all manufactured by Siemens.

MATERIALS AND METHODS

One high sensitivity troponin I assay (TNIH-1) was run using ADVIA Centaur analyzer. The second high sensitivity troponin I assay (TNIH-2) as well as conventional troponin I assay (CTNI) were run using Dimension Vista 1500 analyzer. We analyzed 25 specimens using CTNI, TNIH-1 and TNIH-2 assays for comparison of these assays. Moreover, serum pools prepared from additional specimens containing various amounts of troponin I were further supplemented with biotin to achieve biotin concentrations between 50 and 1000 ng/mL followed by reanalysis using CTNI, TNIH-1 and TNIH-2 assays.

RESULTS

Although both high sensitivity troponin I assays correlated well, there was a significant positive bias with TNIH-2. We observed no significant negative biotin interference at a level up to 250 ng/mL. Highest observed negative bias was 29.7%.

CONCLUSIONS

All three troponin I assays were free from biotin interferences up to a biotin concentration of 250 ng/mL.

摘要

目的

生物素会干扰基于生物素化抗体的免疫测定。我们研究了生物素对西门子公司生产的常规肌钙蛋白 I 和两种高敏肌钙蛋白 I 检测试剂盒的影响。

材料和方法

使用 ADVIA Centaur 分析仪运行一种高敏肌钙蛋白 I 检测试剂盒（TNIH-1）。使用 Dimension Vista 1500 分析仪运行第二种高敏肌钙蛋白 I 检测试剂盒（TNIH-2）和常规肌钙蛋白 I 检测试剂盒（CTNI）。我们使用 CTNI、TNIH-1 和 TNIH-2 检测试剂盒分析了 25 个标本，以比较这些检测试剂盒。此外，我们从含有不同浓度肌钙蛋白 I 的额外标本中制备血清池，然后用生物素将其补充至 50 至 1000ng/mL 的浓度，再使用 CTNI、TNIH-1 和 TNIH-2 检测试剂盒进行重新分析。