来自大鼠睾丸的核糖核酸酶-核糖核酸酶抑制剂复合物。核糖核酸酶抑制剂的纯化。
Ribonuclease-RNAase inhibitor complex from rat testis. Purification of the RNAase inhibitor.
作者信息
Fominaya J M, García-Segura J M, Gavilanes J G
机构信息
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad Complutense, Madrid, Spain.
出版信息
Biochim Biophys Acta. 1988 May 18;954(2):216-23. doi: 10.1016/0167-4838(88)90074-x.
The RNAase inhibitor from rat testis has been purified to homogeneity. The purified protein appeared as a single spot after two-dimensional electrophoresis. The calculated Mr value is 48,000 which coincides with that obtained for the native protein on gel filtration chromatography, thus indicating a single polypeptide chain. The amino acid composition and the characteristics of the inhibitor activity are reported and compared to those of other RNAase inhibitors from mammalian tissues. The naturally occurring ribonuclease-RNAase inhibitor complex from rat testis has also been studied and compared with the rat testis inhibitor-RNAase A as model complex. The ribonuclease released from the natural rat testis complex showed heterogeneity of size. The significance of the rat testis ribonuclease/RNAase inhibitor system is discussed in terms of the important functionality of this organ.
来自大鼠睾丸的核糖核酸酶抑制剂已被纯化至同质。纯化后的蛋白质在二维电泳后呈现为单一斑点。计算得到的Mr值为48,000，这与在凝胶过滤色谱法中对天然蛋白质测得的值一致，从而表明其为单条多肽链。报告了该抑制剂的氨基酸组成及活性特征，并与来自哺乳动物组织的其他核糖核酸酶抑制剂进行了比较。还对来自大鼠睾丸的天然核糖核酸酶 - 核糖核酸酶抑制剂复合物进行了研究，并将其与作为模型复合物的大鼠睾丸抑制剂 - 核糖核酸酶A进行了比较。从天然大鼠睾丸复合物中释放出的核糖核酸酶显示出大小的异质性。根据该器官的重要功能，讨论了大鼠睾丸核糖核酸酶/核糖核酸酶抑制剂系统的意义。
Zotero 插件