Ashun Ebenezer, Toor Umair Ali, Eom Heonseop, Oh Sang-Eun
Department of Biological Environment, Kangwon National University, Chuncheon, Republic of Korea.
Bio Protoc. 2020 Jan 5;10(1):e3470. doi: 10.21769/BioProtoc.3470.
A new direct contact assessment of soil toxicity using sulfur oxidizing bacteria (SOB) is proposed for analyzing the toxicity of soils. The proposed method is based on the ability of SOB to oxidize elemental sulfur to sulfuric acid in the presence of oxygen. Since sulfate ions are produced from sulfur by SOB oxidation activity, changes in electrical conductivity (EC) serve as a proxy to assess toxicity in water. However, in soil medium, EC values are not reliable due to the adsorption of SO ions by soils. Here, we suggest a new parameter which measures oxygen consumption by SOB for 6 hours to assess soil toxicity by using a lubricated glass syringe method. The proposed method is rapid, simple, cost- effective as well as sensitive and capable of assessing direct contact soil toxicity.
提出了一种利用硫氧化细菌（SOB）对土壤毒性进行新的直接接触评估方法，用于分析土壤毒性。该方法基于SOB在有氧条件下将元素硫氧化为硫酸的能力。由于SOB氧化活性使硫产生硫酸根离子，电导率（EC）的变化可作为评估水中毒性的指标。然而，在土壤介质中，由于土壤对硫酸根离子的吸附，EC值并不可靠。在此，我们提出一个新参数，通过润滑玻璃注射器法测量SOB 6小时的耗氧量来评估土壤毒性。该方法快速、简单、经济高效，且灵敏，能够评估土壤直接接触毒性。
