在与真菌PH-1相互作用期间对In5中的成像基因表达动力学进行研究。
Imaging Gene Expression Dynamics in In5 duringInteractions with the Fungus PH-1.
作者信息
Hennessy Rosanna C, Stougaard Peter, Olsson Stefan
机构信息
Section for Microbial Ecology and Biotechnology, Department of Plant and Environmental Sciences, University of Copenhagen, Denmark.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China.
出版信息
Bio Protoc. 2019 Jun 20;9(12):e3264. doi: 10.21769/BioProtoc.3264.
Abstract
Genomics, transcriptomics and metabolomics are powerful technologies for studying microbial interactions. The main drawback of these methods is the requirement for destructive sampling. We have established an alternative but complementary technique based on a microplate system combined with promoter fusions for visualizing gene expression in space and time. Here we provide a protocol for measuring spatial and temporal gene expression of a bacterial reporter strain interacting with a fungus on a solid surface.
摘要
基因组学、转录组学和代谢组学是研究微生物相互作用的强大技术。这些方法的主要缺点是需要进行破坏性取样。我们基于微孔板系统结合启动子融合技术建立了一种替代但互补的技术，用于在空间和时间上可视化基因表达。在此，我们提供了一种用于测量与真菌在固体表面相互作用的细菌报告菌株的空间和时间基因表达的方案。
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Biosynthesis of the antimicrobial cyclic lipopeptides nunamycin and nunapeptin by Pseudomonas fluorescens strain In5 is regulated by the LuxR-type transcriptional regulator NunF.荧光假单胞菌 In5 菌株合成抗菌环状脂肽 nunamycin 和 nunapeptin 由 LuxR 型转录调节因子 NunF 调控。
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