Magno Luiz Alexandre Viana, Collodetti Mélcar, Tenza-Ferrer Helia, Romano-Silva Marco Aurélio
Centro de Tecnologia em Medicina Molecular, Faculdade de Medicina, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, CEP 30130-100, Brazil.
Departamento de Saúde Mental, Faculdade de Medicina, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, CEP 30130-100, Brazil.
Bio Protoc. 2019 Aug 20;9(16):e3337. doi: 10.21769/BioProtoc.3337.
Parkinson's disease is a progressive neurodegenerative movement disorder that happens due to the loss of dopaminergic neurons in the substantia nigra. The deficiency of dopamine in the basal nuclei drives cardinal motor symptoms such as bradykinesia and hypokinesia. The current protocol describes the cylinder test, which is a relatively simple behavioral assessment that evaluates the motor deficits upon unilateral degeneration of the nigrostriatal pathway in experimental models of Parkinson's disease. Since dopamine-depleted mice exhibit the preferential use of the forelimb ipsilateral to the lesion, here researchers perform the cylinder test to investigate the therapeutic effects of antiparkinsonian treatments on the performance of the contralateral (injured) limb.
帕金森病是一种进行性神经退行性运动障碍，其发生是由于黑质中多巴胺能神经元的丧失。基底核中多巴胺的缺乏导致诸如运动迟缓、运动减少等主要运动症状。当前方案描述了圆筒试验，这是一种相对简单的行为评估，用于评估帕金森病实验模型中黑质纹状体通路单侧变性后的运动缺陷。由于多巴胺耗竭的小鼠表现出优先使用损伤侧前肢，因此研究人员在此进行圆筒试验，以研究抗帕金森病治疗对侧（受伤）肢体表现的治疗效果。
