Development of an Efficient Transformation System for Halotolerant Yeast CBS767.

is one of the most osmotolerant and halotolerant yeasts. Further, its association with traditional cheese and meat products imparting special flavors to these products project this yeast with enormous biotechnological potential in the agrofood sector. However, lack of an efficient transformation system in still direct the complementation based assay in mutants for functional analysis of genes. Here, we have described the development of an efficient transformation system for that is based on a histidine auxotrophic recipient strain, DBH9 (generated by UV induced random mutagenesis), and the DhHIS4 gene as the selectable marker. Moreover, the same method has also been employed for gene disruption in by homologous recombination.