Reverte Marta, Fasel Nicolas
Department of Biochemistry, University of Lausanne, Epalinges, Switzerland.
Bio Protoc. 2019 Nov 20;9(22):e3431. doi: 10.21769/BioProtoc.3431.
Leishmaniasis remains a major public health problem worldwide with a prevalence of 12 million, an incidence of 1 million persons, and 350 million people being at risk. Murine models have been largely used for studying the host-pathogen relationship and developing effective chemotherapies against parasites. Thus, preclinical imaging is crucial for monitoring the disease outcome. The aim of this protocol is to quantify parasite burden using bioluminescence imaging. Here, we describe a high-throughput imaging workflow, together with data acquisition and analysis ideal to assess parasite load in mouse models.
利什曼病仍然是全球一个主要的公共卫生问题，全球有1200万人患病，100万人发病，3.5亿人面临风险。鼠类模型在很大程度上已被用于研究宿主与病原体的关系以及开发针对寄生虫的有效化疗方法。因此，临床前成像对于监测疾病结果至关重要。本方案的目的是使用生物发光成像来量化寄生虫负荷。在此，我们描述了一种高通量成像工作流程，以及用于评估小鼠模型中寄生虫负荷的数据采集和分析方法。
