Franco Maribel, Carmena Ana
Developmental Neurobiology Department, Instituto de Neurociencias-Consejo Superior de Investigaciones Científicas/Universidad Miguel Hernández, Sant Joan d'Alacant 03550, Alicante, Spain.
Bio Protoc. 2019 Nov 20;9(22):e3432. doi: 10.21769/BioProtoc.3432.
The positioning and the cleavage plane orientation of mitotic cells in pseudostratified epithelia (PSE) must be tightly regulated since failures in any of these processes might have fatal consequences during development. Here we present a simple method to determine the spindle orientation as well as the positioning of neuroepithelial mitotic cells within the Outer Proliferation Center (OPC) of larval brains.
假复层上皮（PSE）中处于有丝分裂期细胞的定位和分裂平面方向必须受到严格调控，因为这些过程中任何一个出现失误都可能在发育过程中产生致命后果。在此，我们展示一种简单方法，用于确定幼虫脑外增殖中心（OPC）内神经上皮有丝分裂细胞的纺锤体方向以及定位。
