幼虫脑固定组织中纺锤体角度和有丝分裂细胞距离的测量
Measurement of Mitotic Spindle Angle and Mitotic Cell Distance in Fixed Tissue of Larval Brains.
作者信息
Franco Maribel, Carmena Ana
机构信息
Developmental Neurobiology Department, Instituto de Neurociencias-Consejo Superior de Investigaciones Científicas/Universidad Miguel Hernández, Sant Joan d'Alacant 03550, Alicante, Spain.
出版信息
Bio Protoc. 2019 Nov 20;9(22):e3432. doi: 10.21769/BioProtoc.3432.
Abstract
The positioning and the cleavage plane orientation of mitotic cells in pseudostratified epithelia (PSE) must be tightly regulated since failures in any of these processes might have fatal consequences during development. Here we present a simple method to determine the spindle orientation as well as the positioning of neuroepithelial mitotic cells within the Outer Proliferation Center (OPC) of larval brains.
摘要
假复层上皮（PSE）中处于有丝分裂期细胞的定位和分裂平面方向必须受到严格调控，因为这些过程中任何一个出现失误都可能在发育过程中产生致命后果。在此，我们展示一种简单方法，用于确定幼虫脑外增殖中心（OPC）内神经上皮有丝分裂细胞的纺锤体方向以及定位。
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