Basilico Bernadette, Cortese Barbara, Ratano Patrizia, Angelantonio Silvia Di, Ragozzino Davide
Dept of Physiology and Pharmacology, Sapienza University, Rome, Italy.
CNR NANOTEC-Istituto di Nanotecnologia, Dept of Physics, Sapienza University, Rome, Italy.
Bio Protoc. 2019 Apr 20;9(8):e3220. doi: 10.21769/BioProtoc.3220.
Microglia are the resident immune cells of the central nervous system (CNS). In the last year, the improvements in the transgenic mouse technologies and imaging techniques have shed light on microglia functions under physiological conditions. Microglia continuously scan the brain parenchyma with their highly motile processes, maintaining tissue homeostasis and participating in neuronal circuits refinement. Here, we describe a protocol that enables us to perform time-lapse imaging of microglial cells in acute hippocampal slices, making image acquisition possible on an electrophysiology rig equipped with a standard imaging system. Using this approach, we investigated microglial processes scanning abilities under physiological condition in hippocampus.
小胶质细胞是中枢神经系统（CNS）中的常驻免疫细胞。去年，转基因小鼠技术和成像技术的进步揭示了生理条件下小胶质细胞的功能。小胶质细胞通过其高度活跃的突起持续扫描脑实质，维持组织稳态并参与神经回路的优化。在这里，我们描述了一种方案，该方案使我们能够对急性海马切片中的小胶质细胞进行延时成像，从而可以在配备标准成像系统的电生理装置上进行图像采集。使用这种方法，我们研究了海马体在生理条件下小胶质细胞突起的扫描能力。
