Department of Obstetrics and Gynecology, Regional Training Research Hospital, Erzurum, Turkey.
Gen Physiol Biophys. 2021 Jan;40(1):71-78. doi: 10.4149/gpb_2020039.
We investigated the effect of ATP's protection against possible bevacizumab-induced ovarian damage and reproductive dysfunction in female albino Wistar rats. A total of 42 rats, 36 females, and 6 males were used in the experiment. Normal saline (0.9% NaCl) was injected as a solvent to the Bevacizumab (BVZ; n = 12) and Control (n = 6) groups. 25 mg/kg ATP was injected intraperitoneally (i.p.) to the ATP + bevacizumab (ABZ; n = 12) group. One hour after ATP and solvent administration, 10 mg/kg bevacizumab was i.p. injected to the ABZ and BVZ groups. Bevacizumab was administered once a day every two weeks; ATP was administered one a day for 30 days. At the end of this period, six rats from each group were sacrificed with high dose of anesthesia (thiopental sodium 50 mg/kg) and biochemical and histopathological examinations were performed in ovarian tissues. Mature male rats were kept in the laboratory for two months to breed the remaining female animals. The values showed that the oxidant parameters increased in the ovarian tissue of the BVZ group compared to the healthy controls and the ABZ group, while antioxidant parameters decreased. The number of breeding animals was significantly decreased in the BVZ group compared to the Control and the ABZ groups. This result suggests that ATP may be effective in preventing oxidative damage to the ovaries and infertility induced by bevacizumab.
我们研究了 ATP 对可能由贝伐珠单抗引起的卵巢损伤和雌性白化 Wistar 大鼠生殖功能障碍的保护作用。实验共使用了 42 只大鼠，其中 36 只为雌性，6 只为雄性。贝伐珠单抗（BVZ；n = 12）和对照组（n = 6）注射生理盐水（0.9% NaCl）作为溶剂。ATP（25 mg/kg）腹腔注射到 ATP + 贝伐珠单抗（ABZ；n = 12）组。ABZ 和 BVZ 组在 ATP 和溶剂给药后 1 小时，腹腔注射 10 mg/kg 贝伐珠单抗。贝伐珠单抗每两周给药一次，每天一次；ATP 每天给药一次，共 30 天。在这个周期结束时，每组 6 只大鼠用大剂量麻醉（硫喷妥钠 50 mg/kg）处死，并对卵巢组织进行生化和组织病理学检查。成熟雄性大鼠在实验室中饲养两个月，以繁殖其余的雌性动物。结果表明，与健康对照组和 ABZ 组相比，BVZ 组卵巢组织中的氧化参数增加，抗氧化参数减少。BVZ 组的繁殖动物数量明显少于对照组和 ABZ 组。这一结果表明，ATP 可能有效预防贝伐珠单抗引起的卵巢氧化损伤和不孕。
