State Key Laboratory for Conservation and Utilization of Subtropical gro-bioresources, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
State Key Laboratory for Conservation and Utilization of Subtropical gro-bioresources, College of Life Science and Technology, Guangxi University, Nanning, Guangxi, China.
Methods Mol Biol. 2021;2297:61-74. doi: 10.1007/978-1-0716-1370-2_7.
The circadian clock is a self-sustaining 24 h timekeeper which enables plants to anticipate periodic environmental changes and optimize the biological activities for most beneficial time during day/night cycles. As in many organisms, the sustained circadian rhythmicity in plant relies on network of transcriptional/translational feedback loops (TTFLs) of transcription factors at the core of the oscillator. Over the past years, ChIP-seq has become an indispensable method to uncover the clock network through identifications of circadian transcription factors binding sites on a genome-wide scale. Here, we show how to use ChIP-seq to analyze the occupancy of circadian transcription factor in Arabidopsis. In addition, we briefly describe some modifications of protocol applied to rice (Oryza sativa).
生物钟是一种自我维持的 24 小时计时系统，使植物能够预测周期性的环境变化，并在日夜周期中优化最有利的生物活动时间。与许多生物一样，植物中持续的昼夜节律依赖于转录因子的转录/翻译反馈环（TTFL）网络，该网络是振荡器的核心。在过去的几年中，ChIP-seq 已成为通过在全基因组范围内识别生物钟转录因子结合位点来揭示生物钟网络的不可或缺的方法。在这里，我们展示如何使用 ChIP-seq 来分析拟南芥中生物钟转录因子的占据情况。此外，我们还简要描述了应用于水稻（Oryza sativa）的一些协议修改。
