Biochemical Engineering College, Beijing Union University, Beijing, China.
Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
Methods Mol Biol. 2021;2297:95-103. doi: 10.1007/978-1-0716-1370-2_10.
Seedling deetiolation is a hallmark of the photomorphogenic response, and successful conversion of protochlorophyllide (Pchlide) into chlorophyllide during initial light exposure is critical for plant survival and growth. Here we describe the seedling deetiolation process of two typical mutants pif3 and flu by analysis of the cotyledons greening, Pchlide content, and reactive oxygen species (ROS) production and summarize a set of general methods for the research of seedling greening.
幼苗去黄化是光形态建成反应的一个标志，在初始光照下成功地将原叶绿素ide（Pchlide）转化为叶绿素ide 对植物的生存和生长至关重要。在这里，我们通过分析子叶变绿、Pchlide 含量以及活性氧（ROS）产生来描述两个典型突变体 pif3 和 flu 的幼苗去黄化过程，并总结了一套用于幼苗变绿研究的一般方法。
