原核生物系统用于研究蓝光依赖性隐花色素光体的形成。

Protoplast System for Studying Blue-Light-Dependent Formation of Cryptochrome Photobody.

机构信息

Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.

出版信息

Methods Mol Biol. 2021;2297:105-113. doi: 10.1007/978-1-0716-1370-2_11.

Abstract

Cryptochromes (CRYs) belong to an ancient and conserved class of blue-light receptor regulating circadian clock and development in animals and plants. Arabidopsis CRY2 form physiologically active homodimers in response to blue light treatment and further oligomerize into photobodies, which are expected to be the foci harboring protein interaction, phosphorylation, and ubiquitination. Here we describe two efficient methods developed to test the formation of blue-light-dependent photobodies of CRY-GFP fusing proteins using the mesophyll protoplasts of Arabidopsis or soybean, respectively.

摘要

隐花色素（CRYs）属于一类古老而保守的蓝光受体，在动植物中调节生物钟和发育。拟南芥 CRY2 在蓝光处理下形成生理活性的同源二聚体，进一步寡聚形成光体，预计这些光体是含有蛋白相互作用、磷酸化和泛素化的焦点。在这里，我们分别描述了两种使用拟南芥或大豆的叶肉原生质体来测试 CRY-GFP 融合蛋白形成蓝光依赖性光体的有效方法。