Zhang Jingyi, He Shengbo
Department of Cell and Developmental Biology, John Innes Centre, Norwich, UK.
Methods Mol Biol. 2021;2297:141-146. doi: 10.1007/978-1-0716-1370-2_14.
Co-immunoprecipitation (CoIP) assay has been used as a powerful and routine technique to detect in vivo protein-protein interactions. Not only can it probe stable interactions, but also it is applicable for semiquantitative and inducible protein associations. Here we describe the protocol for detecting blue light-dependent protein interactions, particularly for blue light receptor cryptochrome-mediated complex formation. In addition, we present some notes which may be helpful for common Co-IP study as well.
免疫共沉淀（CoIP）分析已被用作一种强大的常规技术来检测体内蛋白质-蛋白质相互作用。它不仅可以探测稳定的相互作用，还适用于半定量和可诱导的蛋白质结合。在这里，我们描述了检测蓝光依赖性蛋白质相互作用的方案，特别是针对蓝光受体隐花色素介导的复合物形成。此外，我们还给出了一些可能对常见的免疫共沉淀研究也有帮助的注意事项。
