Yudin A I, Cherr G N, Katz D F
Department of Obstetrics and Gynecology, School of Medicine, University of California, Davis 95616.
Cell Tissue Res. 1988 Mar;251(3):555-64. doi: 10.1007/BF00214003.
Hamster oocyte-cumulus complexes (OCC), with and without sperm, were structurally analyzed by light- and electron microscopy using freeze substitution. This method has yielded a clear picture of the extracellular oocyte investments, the cumulus cell matrix and the zona pellucida. The cumulus matrix has an overall homogeneous fibrillar structure which appears to attach to cumulus cells at their filopodial extensions. The matrix also extends into the outer regions of the zona pellucida. The zona pellucida has a distinct porous configuration throughout its entire structure. During gamete interaction experiments, capacitated hamster sperm with ultrastructurally intact acrosomes were found throughout the matrix. Sperm had dramatic effects on the matrix, resulting in compression and stretching. Sperm found on the zona pellucida had initiated or completed the acrosome reaction. During the initial stages of the acrosome reaction, the matrix was in contact with the sperm. At later stages of the acrosome reaction, there was a complete loss of matrix material in regions near the sperm.
采用冷冻置换法，通过光学显微镜和电子显微镜对有无精子的仓鼠卵母细胞-卵丘复合体（OCC）进行结构分析。该方法清晰呈现了卵母细胞的细胞外覆盖物、卵丘细胞基质和透明带的情况。卵丘基质具有整体均匀的纤维状结构，似乎在其丝状伪足延伸处附着于卵丘细胞。基质还延伸至透明带的外部区域。透明带在其整个结构中具有独特的多孔构型。在配子相互作用实验中，在整个基质中发现了超微结构完整顶体的获能仓鼠精子。精子对基质有显著影响，导致其压缩和拉伸。在透明带上发现的精子已启动或完成顶体反应。在顶体反应的初始阶段，基质与精子接触。在顶体反应的后期，精子附近区域的基质物质完全消失。
