Wu Z Y, Hu X B
Department of Orthopedics, First Hospital, Chongqing University of Medical Sciences, Sichuan, People's Republic of China.
Clin Orthop Relat Res. 1988 May(230):229-36.
Porcine bone morphogenetic protein (pBMP) was separated and purified from porcine bone by repeated solubilization and precipitation of the protein with different concentrations of urea and GuHCl. The molecular weight of pBMP was determined to be 19.7 k. The property of inductive osteogenesis of pBMP caused new bone formation in muscular and subcutaneous tissues of strain No. 615 mice. Osteoid tissues were observed as early as ten days after implantation of pBMP, and lamellated new bones with marrow cavities were first found on the 15th day of implantation. A gradual transformation of mesenchymal-type cells into active chondroblasts and osteoblasts and finally into heterotopic new bone was observed and studied histologically and ultrastructurally.
通过用不同浓度的尿素和盐酸胍反复溶解和沉淀蛋白质，从猪骨中分离并纯化出猪骨形态发生蛋白（pBMP）。测定pBMP的分子量为19.7k。pBMP的诱导成骨特性在615品系小鼠的肌肉和皮下组织中引起了新骨形成。早在植入pBMP十天后就观察到类骨质组织，在植入第15天首次发现带有骨髓腔的板层状新骨。从组织学和超微结构上观察并研究了间充质型细胞逐渐转变为活跃的成软骨细胞和成骨细胞，最终形成异位新骨的过程。
