从培养的哺乳动物细胞中分离脂筏及其脂质组学分析。
Isolation of Lipid Rafts from Cultured Mammalian Cells and Their Lipidomics Analysis.
作者信息
Mukhamedova Nigora, Huynh Kevin, Low Hann, Meikle Peter J, Sviridov Dmitri
机构信息
Baker Heart and Diabetes Institute, Melbourne, VIC, Australia.
出版信息
Bio Protoc. 2020 Jul 5;10(13):e3670. doi: 10.21769/BioProtoc.3670.
Abstract
Lipid rafts are distinct liquid-ordered domains of plasma membranes of most eukaryotic cells providing platform for signaling pathways. Lipid composition of rafts is critical for their structural integrity and for regulation of signaling pathways originating from rafts. Here we provide a protocol to isolate lipid rafts from cultured human and animal cells and comprehensively analyse their lipid composition.
摘要
脂筏是大多数真核细胞质膜中独特的液相有序结构域，为信号通路提供平台。脂筏的脂质组成对于其结构完整性以及源自脂筏的信号通路的调节至关重要。在此，我们提供了一种从培养的人和动物细胞中分离脂筏并全面分析其脂质组成的方法。
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