Clegg James M, Pratt Thomas
Simons Initiative for the Developing Brain, University of Edinburgh, Edinburgh EH8 9XD, UK.
Bio Protoc. 2020 Jul 5;10(13):e3674. doi: 10.21769/BioProtoc.3674.
Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays and bead culture assays.
器官型切片培养是探索哺乳动物大脑胚胎发育的一种强大技术。在本方案中，我们描述了一种我们用于两组实验的基本切片培养技术：轴突导向移植试验和珠子培养试验。
