胚胎小鼠脑的器官型切片培养
Organotypic Slice Culture of the Embryonic Mouse Brain.
作者信息
Clegg James M, Pratt Thomas
机构信息
Simons Initiative for the Developing Brain, University of Edinburgh, Edinburgh EH8 9XD, UK.
出版信息
Bio Protoc. 2020 Jul 5;10(13):e3674. doi: 10.21769/BioProtoc.3674.
Abstract
Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays and bead culture assays.
摘要
器官型切片培养是探索哺乳动物大脑胚胎发育的一种强大技术。在本方案中，我们描述了一种我们用于两组实验的基本切片培养技术：轴突导向移植试验和珠子培养试验。
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