Pezzoni Magdalena, Pizarro Ramón A, Costa Cristina S
Dpto. de Radiobiología, Comisión Nacional de Energía Atómica, General San Martín, Argentina.
Bio Protoc. 2020 Sep 20;10(18):e3762. doi: 10.21769/BioProtoc.3762.
is a human pathogen capable to form robust biofilms. biofilms represent a serious problem because of the adverse effects on human health and industry, from sanitary and economic points of view. Typical strategies to break down biofilms have been long used, such as the use of disinfectants or antibiotics, but also, according to their high resistance to standard antimicrobial approaches, alternative strategies employing photocatalysis or control of biofilm formation by modifying surfaces, have been proposed. Colony forming units (cfu) counting and live/dead staining, two classic techniques used for biofilm quantification, are detailed in this work. Both methods assess cell viability, a key factor to analyze the microbial susceptibility to given treatment, then, they represent a good approach for evaluation of an antibiofilm strategy.
是一种能够形成坚固生物膜的人类病原体。从卫生和经济角度来看，生物膜由于对人类健康和工业产生不利影响而成为一个严重问题。长期以来一直使用典型的分解生物膜的策略，例如使用消毒剂或抗生素，但由于生物膜对标准抗菌方法具有高抗性，也有人提出采用光催化或通过修饰表面来控制生物膜形成的替代策略。本工作详细介绍了用于生物膜定量的两种经典技术——菌落形成单位（cfu）计数和活/死染色。这两种方法都评估细胞活力，而细胞活力是分析微生物对给定处理敏感性的关键因素，因此，它们是评估抗生物膜策略的良好方法。
