基于线粒体和基因的湖南省蛇类分离株的遗传多样性

[Genetic diversity of isolates from snakes in Hunan Province based on mitochondrial and genes].

作者信息

Gong T F, He J L, Tan L, Xie L X, Liu W

机构信息

College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.

出版信息

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2021 Jan 18;33(1):35-39. doi: 10.16250/j.32.1374.2020305.

DOI:10.16250/j.32.1374.2020305

PMID:33660472

Abstract

OBJECTIVE

To investigate the genetic diversity and phylogenetic relationship of isolates from snakes in Hunan Province.

METHODS

The partial mitochondrial NADH dehydrogenase subunit 4 () and NADH dehydrogenase subunit 5 () genes were amplified using a PCR assay in 7 isolates from snakes in Hunan Province and the amplification product was sequenced. The homology and genetic evolution were investigated using the software DNAMAN 7.0, MegAlign, DnaSP 5.0 and MEGA 5.0.

RESULTS

The and gene sequences were approximately 578 bp and 484 bp in length in the 7 isolates from Hunan Province, and the percentages of genetic variations were 0 to 2.8% and 0 to 0.8%, respectively. There were 4 haplotypes detected in both the and genes, with global haplotype diversities of 0.810 ± 0.016 and 0.905 ± 0.011, nucleotide diversities of 0.006 ± 0.005 and 0.004 ± 0.003, and mean nucleotide variations of 3.960 and 1.905, respectively. Phylogenetic analysis showed that all 7 isolates from snakes in Hunan Province were clustered into the same branch with isolates from different regions/hosts in the world, which belonged to , which were close to and far from other tapeworms.

CONCLUSIONS

There is a low genetic variation in snake-derived isolates from Hunan Province, and both and genes may be potential molecular genetic markers for identification of .

摘要

目的

研究湖南省蛇源分离株的遗传多样性及系统发育关系。

方法

采用聚合酶链反应（PCR）法扩增湖南省7株蛇源分离株的线粒体烟酰胺腺嘌呤二核苷酸脱氢酶亚基4（ND4）和烟酰胺腺嘌呤二核苷酸脱氢酶亚基5（ND5）基因部分片段，并对扩增产物进行测序。使用DNAMAN 7.0、MegAlign、DnaSP 5.0和MEGA 5.0软件对其同源性及遗传进化进行分析。

结果

湖南省7株蛇源分离株的ND4和ND5基因序列长度分别约为578 bp和484 bp，遗传变异率分别为0%至2.8%和0%至0.8%。ND4和ND5基因均检测到4种单倍型，全球单倍型多样性分别为0.810±0.016和0.905±0.011，核苷酸多样性分别为0.006±0.005和0.004±0.003，平均核苷酸差异分别为3.960和1.905。系统发育分析表明，湖南省7株蛇源分离株与世界不同地区/宿主的分离株聚为同一分支，均属于某一类别，与某一类别关系密切，与其他绦虫距离较远。