促进大肠杆菌中稳定核糖核酸降解的F质粒基因的遗传定位。
Genetic mapping of the F plasmid gene that promotes degradation of stable ribonucleic acid in Escherichia coli.
作者信息
Ohnishi Y, Iguma H, Ono T, Nagaishi H, Clark A J
出版信息
J Bacteriol. 1977 Dec;132(3):784-9. doi: 10.1128/jb.132.3.784-789.1977.
Abstract
F+ Escherichi coli cells that contain an srnA mutant allele degrade their stable ribonucleic acid (RNA) extensively after RNA synthesis is blocked at 42 degrees C. The relevant gene promoting degradation of stable RNA, srnB+, or its promoter was mapped between 1.7 and 2.8 kilobases on the F plasmid by using deleted F' plasmids and chimeric plasmids composed of pSC101 and fragments of F plasmid.
摘要
含有srnA突变等位基因的F⁺大肠杆菌细胞在42℃下RNA合成受阻后，会大量降解其稳定的核糖核酸（RNA）。通过使用缺失的F'质粒以及由pSC101和F质粒片段组成的嵌合质粒，将促进稳定RNA降解的相关基因srnB⁺或其启动子定位在F质粒上1.7至2.8千碱基之间。
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