J Am Chem Soc. 2021 Mar 17;143(10):3671-3676. doi: 10.1021/jacs.0c11699. Epub 2021 Mar 4.
This paper describes the light-directed functionalization of anisotropic gold nanoparticles with different thiolated-DNA oligomer (oligo) sequences. The starting nanoconstructs are gold nanostars (AuNS) uniformly grafted with one oligo sequence that are then exposed to fs-laser pulses at the plasmon resonance of the branches. The excitation selectively cleaves Au-S bonds at the tips of the branches to create vacant areas for functionalization with a different thiolated oligo sequence. Nanoconstructs synthesized by this approach present one oligo sequence on the AuNS body and branches and a different sequence at the tips. This process enables the formation of nanoparticle superstructures consisting of AuNS cores and small Au satellite nanoparticles at controlled locations after DNA hybridization. Our strategy enables selective oligo presentation at the single-particle level and opens prospects for sophisticated design of nanoscale assemblies that are important in a wide range of biological applications.
本文描述了各向异性金纳米星（AuNS）通过不同巯基化 DNA 寡聚物（oligo）序列的定向光功能化。起始纳米结构是均勻接枝有一个 oligo 序列的 AuNS，然后在支链的等离子体共振处用 fs 激光脉冲照射。激发选择性地在支链的尖端切割 Au-S 键，为功能化创造不同的巯基化 oligo 序列的空位。通过这种方法合成的纳米结构在 AuNS 体和支链上呈现一个 oligo 序列，在尖端呈现不同的序列。该过程使得在 DNA 杂交后，能够在特定位置形成由 AuNS 核和小 Au 卫星纳米颗粒组成的纳米颗粒超结构。我们的策略能够在单颗粒水平上选择性地呈现 oligo，并为纳米级组装的复杂设计开辟了前景，这在广泛的生物应用中非常重要。
